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P4HB ELISA Kit

P4HB Reaktivität: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Produktnummer ABIN6730762
  • Target Alle P4HB ELISA Kits anzeigen
    P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))
    Reaktivität
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Human P4HB ELISA Kit.
    Proben
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Spezifität
    This ELISA antibody pair recognizes Human P4HB.
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product P4HB ELISA Kit
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Informationen zur Lagerung
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Haltbarkeit
    6 months
  • Target Alle P4HB ELISA Kits anzeigen
    P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))
    Andere Bezeichnung
    P4HB (P4HB Produkte)
    Synonyme
    DSI ELISA Kit, ERBA2L ELISA Kit, GIT ELISA Kit, P4Hbeta ELISA Kit, PDI ELISA Kit, PDIA1 ELISA Kit, PHDB ELISA Kit, PO4DB ELISA Kit, PO4HB ELISA Kit, PROHB ELISA Kit, PDA2 ELISA Kit, PDIP ELISA Kit, PDIR ELISA Kit, ERp59 ELISA Kit, Pdia1 ELISA Kit, Thbp ELISA Kit, p55 ELISA Kit, XPDIp ELISA Kit, pdi ELISA Kit, 1810041F13Rik ELISA Kit, AI661267 ELISA Kit, Pdip ELISA Kit, Pdipl ELISA Kit, p58 ELISA Kit, prolyl 4-hydroxylase subunit beta ELISA Kit, protein disulfide isomerase family A member 2 ELISA Kit, prolyl 4-hydroxylase, beta polypeptide ELISA Kit, protein disulfide isomerase family A member 2 L homeolog ELISA Kit, protein disulfide isomerase associated 2 ELISA Kit, prolyl 4-hydroxylase subunit beta L homeolog ELISA Kit, protein disulfide-isomerase ELISA Kit, P4HB ELISA Kit, PDIA2 ELISA Kit, P4hb ELISA Kit, pdia2.L ELISA Kit, Pdia2 ELISA Kit, p4hb.L ELISA Kit, LOC8281065 ELISA Kit
    UniProt
    P07237
    Pathways
    Maintenance of Protein Location, Cell RedoxHomeostasis, Lipid Metabolism
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