ALT ELISA Kit

Produktnummer ABIN6730944

Produktdetails ALT ELISA Kit

Target: ALT (Alanine Aminotransferase (ALT))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.78 ng/mL - 50 ng/mL

Untere Nachweisgrenze: 0.78 ng/mL

Applikation: ELISA

Verwendungszweck: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ALT in rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates.

We offer validation data (WB) for the kit components. So you can be sure to order a reliable ELISA kit product composed of high quality reagents.

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Alanine Aminotransferase (ALT)

Kreuzreaktivität (Details): No significant cross-reactivity or interference between Alanine Aminotransferase (ALT) and analogues was observed.

Sensitivität: 0.27 ng/mL

Bestandteile:

• Pre-coated, ready to use 96-well strip plate, flat buttom
• Plate sealer for 96 wells
• Reference Standard
• Standard Diluent
• Detection Reagent A
• Detection Reagent B
• Assay Diluent A
• Assay Diluent B
• Reagent Diluent (if Detection Reagent is lyophilized)
• TMB Substrate
• Stop Solution
• Wash Buffer (30 x concentrate)
• Instruction manual

Anwendungsinformationen

Kommentare:

Information on standard material:
The standard might be recombinant protein or natural protein, that will depend on the specific kit. Moreover, the expression system is E.coli or yeast or mammal cell. There is 0.05% proclin 300 in the standard as preservative.

Information on reagents:
The stop solution used in the kit is sulfuric acid with concentration of 1 mol/L. And the wash solution is TBS. The standard diluent contains 0.02 % sodium azide, assay diluent A and assay diluent B contain 0.01% sodium azide. Some kits can contain is BSA in them.

Information on antibodies:
The provided antibodies and their host vary in different kits.

Probenmenge: 100 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards,
2. Add 100μL standard or sample to each well. Incubate 1 hours at 37 °C,
3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37 °C,
4. Aspirate and wash 3 times,
5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C,
6. Aspirate and wash 5 times,
7. Add 90μL Substrate Solution. Incubate 10-20 minutes at 37 °C,
8. Add 50μL Stop Solution. Read at 450nm immediately.

Aufbereitung der Reagenzien:

1. Bring all kit components and samples to room temperature (18-25 °C) before use. If the kit will not be used up in one time, please only take out strips and reagents for present experiment, and leave the remaining strips and reagents in required condition.
2. Standard - Reconstitute the Standard with 1.0 mL of Standard Diluent, keep for 10 minutes at room temperature, shake gently (not to foam). The concentration of the standard in the stock solution is 250 ng/mL. Firstly dilute the stock solution to 50 ng/mL and the diluted standard serves as the highest standard (50 ng/mL). Then prepare 7 tubes containing 0.5 mL Standard Diluent and use the diluted standard to produce a double dilution series. Mix each tube thoroughly before the next transfer. Set up 7 points of diluted standard such as 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL, and the last microcentrifuge tube with Standard Diluent is the blank as 0 ng/mL.
3. Detection Reagent A and Detection Reagent B - If lyophilized reconstitute the Detection Reagent A with 150μL of Reagent Diluent, keep for 10 minutes at room temperature, shake gently (not to foam). Briefly spin or centrifuge the stock Detection A and Detection B before use. Dilute them to the working concentration 100-fold with Assay Diluent A and B, respectively.
4. Wash Solution - Dilute 20 mL of Wash Solution concentrate (30x) with 580 mL of deionized or distilled water to prepare 600 mL of Wash Solution (1x).
5. TMB substrate - Aspirate the needed dosage of the solution with sterilized tips and do not dump the residual solution into the vial again.

Note:

1. Making serial dilution in the wells directly is not permitted.
2. Prepare standards within 15 minutes before assay. Please do not dissolve the reagents at 37 °C directly.
3. Please carefully reconstitute Standards or working Detection Reagent A and B according to the instruction, and avoid foaming and mix gently until the crystals are completely dissolved. To minimize imprecision caused by pipetting, use small volumes and ensure that pipettors are calibrated. It is recommended to suck more than 10μL for one pipetting.
4. The reconstituted Standards, Detection Reagent A and Detection Reagent B can be used only once.
5. If crystals have formed in the Wash Solution concentrate (30x), warm to room temperature and mix gently until the crystals are completely dissolved.
6. Contaminated water or container for reagent preparation will influence the detection result.

Aufbereitung der Proben:

• It is recommended to use fresh samples without long storage, otherwise protein degradation and denaturationmay occur in these samples, leading to false results. Samples should therefore be stored for a short periodat 2 - 8 °C or aliquoted at -20 °C (≤1 month) or -80 °C (≤ 3 months). Repeated freeze-thawcycles should be avoided. Prior to assay, the frozen samples should be slowly thawed and centrifuged toremove precipitates.
• If the sample type is not specified in the instructions, a preliminary test is necessary to determinecompatibility with the kit.
• If a lysis buffer is used to prepare tissue homogenates or cell culture supernatant, there is a possibilityof causing a deviation due to the introduced chemical substance.The recommended dilution factor is for reference only.
• Please estimate the concentration of the samples before performing the test. If the values are not in therange of the standard curve, the optimal sample dilution for the particular experiment has to be determined.Samples should then be diluted with PBS (pH =7.0-7.2).

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level of target were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level of target were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV < 10%
Inter-Assay: CV < 12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Vorsichtsmaßnahmen: The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung:

1. For unopened kit: All reagents should be stored according to the labels on the vials. The Standard, Detection Reagent A, Detection Reagent B, and 96-well Strip Plate should be stored at -20 °C upon receipt, while the other reagents should be stored at 4 °C.
2. For opened kits: the remaining reagents must be stored according to the above storage conditions. In addition, please return the unused wells to the foil pouch containing the desiccant and seal the foil pouch with the zipper.

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Haltbarkeit: 6 months

Referenzen für ALT ELISA Kit (ABIN6730944)

Taskin, Erdoğan, Yiğittürk, Günenç, Erbaş: "Antifibrotic Effect of Lactulose on a Methotrexate-Induced Liver Injury Model." in: Gastroenterology research and practice, Vol. 2017, pp. 7942531, (2017) (PubMed).

Feng, Wang, Zhang, Gao, Tao, Ge, Zhu, Bi: "Modulation of gut microbiota contributes to curcumin-mediated attenuation of hepatic steatosis in rats." in: Biochimica et biophysica acta, Vol. 1861, Issue 7, pp. 1801-1812, (2017) (PubMed).

Bakr, El-Naa, Zaghloul, Omar: "Profile of bioactive compounds in Nymphaea alba L. leaves growing in Egypt: hepatoprotective, antioxidant and anti-inflammatory activity." in: BMC complementary and alternative medicine, Vol. 17, Issue 1, pp. 52, (2017) (PubMed).

Tacheci, Edakkanambeth Varayil, Zelinkova, Kopacova, Zavoral: "Advanced Studies in Clinical and Experimental Research in Gastroenterology." in: Gastroenterology research and practice, Vol. 2016, pp. 4253143, (2016) (PubMed).

Charehsaz, Hougaard, Sipahi, Ekici, Kaspar, Culha, Bucurgat, Aydin: "Effects of developmental exposure to silver in ionic and nanoparticle form: A study in rats." in: Daru : journal of Faculty of Pharmacy, Tehran University of Medical Sciences, Vol. 24, Issue 1, pp. 24, (2016) (PubMed).

Ding, Zhang, Dong, Ding, Huang, Zhu, Hutchinson, Gao, Zhang: "Adiponectin protects the rats liver against chronic intermittent hypoxia induced injury through AMP-activated protein kinase pathway." in: Scientific reports, Vol. 6, pp. 34151, (2016) (PubMed).

Polat, Cerrah, Albayrak, Ipek, Arabul, Aslan, Yilmaz: "Assessing the Effect of Leptin on Liver Damage in Case of Hepatic Injury Associated with Paracetamol Poisoning." in: Gastroenterology research and practice, Vol. 2015, pp. 357360, (2015) (PubMed).

Yayla, Halici, Unal, Bayir, Akpinar, Gocer: "Protective effect of Et-1 receptor antagonist bosentan on paracetamol induced acute liver toxicity in rats." in: European journal of pharmacology, Vol. 726, pp. 87-95, (2014) (PubMed).

Si, Ge, Zhuang, Wang, Chen, Gong: "Adrenocorticotropic hormone ameliorates acute kidney injury by steroidogenic-dependent and -independent mechanisms." in: Kidney international, Vol. 83, Issue 4, pp. 635-46, (2013) (PubMed).

Mold, Steinbauer, Wunder, Small: "Outpatient multidisciplinary geriatric assessment I." in: The Journal of the Oklahoma State Medical Association, Vol. 80, Issue 6, pp. 367-71, (1987) (PubMed).

Details zu ALT

Target: ALT (Alanine Aminotransferase (ALT))

Abstract: ALT Produkte

Synonyme: AAT1 ELISA Kit, ALT1 ELISA Kit, GPT1 ELISA Kit, GPT ELISA Kit, Gpt1 ELISA Kit, An11g02620 ELISA Kit, AO090003000164 ELISA Kit, 233.t00009 ELISA Kit, 24.t00016 ELISA Kit, 1300007J06Rik ELISA Kit, 2310022B03Rik ELISA Kit, ALT ELISA Kit, Gpt-1 ELISA Kit, AU021132 ELISA Kit, Dpagt2 ELISA Kit, Gnpta ELISA Kit, gpt ELISA Kit, ALANINE AMINOTRANSFERAS ELISA Kit, AtAlaAT1 ELISA Kit, AtAlaATc ELISA Kit, T13M22.3 ELISA Kit, T13M22_3 ELISA Kit, alanine aminotransferas ELISA Kit, glutamic--pyruvic transaminase ELISA Kit, glutamic-pyruvate transaminase (alanine aminotransferase) ELISA Kit, alanine aminotransferase ELISA Kit, alanine aminotransferase 1 ELISA Kit, glutamic pyruvic transaminase, soluble ELISA Kit, dolichyl-phosphate (UDP-N-acetylglucosamine) acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase) ELISA Kit, alanine aminotransferase 2-like ELISA Kit, Alanine aminotransferase 1, mitochondrial ELISA Kit, GPT ELISA Kit, Gpt ELISA Kit, Tb927.1.3950 ELISA Kit, ANI_1_368094 ELISA Kit, AOR_1_284154 ELISA Kit, ALAT_1 ELISA Kit, ALAAT1 ELISA Kit, EHI_159710 ELISA Kit, EHI_096750 ELISA Kit, LOC5572541 ELISA Kit, ALAT ELISA Kit, AAT2 ELISA Kit, Dpagt1 ELISA Kit, LOC100537633 ELISA Kit, AlaAT1 ELISA Kit

UniProt: P25409