CD300LB ELISA Kit

Produktnummer ABIN6810490

Produktdetails CD300LB ELISA Kit

Target: CD300LB (TREM5) (Triggering Receptor Expressed On Myeloid Cells 5 (TREM5))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Mouse CD300b/LMIR5 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes Mouse CD300b.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Haltbarkeit: 6 months

Details zu CD300LB

Target: CD300LB (TREM5) (Triggering Receptor Expressed On Myeloid Cells 5 (TREM5))

Andere Bezeichnung: CD300b / LMIR5 (TREM5 Produkte)

Synonyme: CLM-7 ELISA Kit, Clm7 ELISA Kit, Gm253 ELISA Kit, LMIR5 ELISA Kit, CD300b ELISA Kit, CLM7 ELISA Kit, CMRF35-A2 ELISA Kit, IREM-3 ELISA Kit, IREM3 ELISA Kit, TREM-5 ELISA Kit, TREM5 ELISA Kit, CD300 molecule like family member B ELISA Kit, CD300 molecule like family member b ELISA Kit, triggering receptor expressed on myeloid cells 5 ELISA Kit, Cd300lb ELISA Kit, CD300LB ELISA Kit, Trem5 ELISA Kit

UniProt: Q3U497