CADM1 ELISA Kit

Produktnummer ABIN6810493

Produktdetails CADM1 ELISA Kit

Target: CADM1 (Cell Adhesion Molecule 1 (CADM1))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Mouse IGSF4A/SynCAM1 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes Mouse IGSF4A.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Haltbarkeit: 6 months

Details zu CADM1

Target: CADM1 (Cell Adhesion Molecule 1 (CADM1))

Andere Bezeichnung: IGSF4A (CADM1 Produkte)

Synonyme: BL2 ELISA Kit, IGSF4 ELISA Kit, IGSF4A ELISA Kit, NECL2 ELISA Kit, Necl-2 ELISA Kit, RA175 ELISA Kit, ST17 ELISA Kit, SYNCAM ELISA Kit, TSLC1 ELISA Kit, sTSLC-1 ELISA Kit, sgIGSF ELISA Kit, synCAM1 ELISA Kit, bl2 ELISA Kit, igsf4 ELISA Kit, igsf4a ELISA Kit, necl-2 ELISA Kit, necl2 ELISA Kit, ra175 ELISA Kit, sgigsf ELISA Kit, st17 ELISA Kit, stslc-1 ELISA Kit, syncam ELISA Kit, syncam1 ELISA Kit, tslc1 ELISA Kit, xnecl2 ELISA Kit, 2900073G06Rik ELISA Kit, 3100001I08Rik ELISA Kit, AI987920 ELISA Kit, Bl2 ELISA Kit, Igsf4 ELISA Kit, Igsf4a ELISA Kit, Necl2 ELISA Kit, RA175A ELISA Kit, RA175B ELISA Kit, RA175C ELISA Kit, RA175N ELISA Kit, SgIGSF ELISA Kit, SynCam ELISA Kit, Tslc1 ELISA Kit, SynCAM1 ELISA Kit, cell adhesion molecule 1 ELISA Kit, cell adhesion molecule 1 L homeolog ELISA Kit, CADM1 ELISA Kit, cadm1.L ELISA Kit, Cadm1 ELISA Kit

UniProt: Q8R5M8

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Activated T Cell Proliferation