FRS2 ELISA Kit

Produktnummer ABIN6810504

Produktdetails FRS2 ELISA Kit

Target: FRS2 (Fibroblast Growth Factor Receptor Substrate 2 (FRS2))

Bindungsspezifität: pTyr

Reaktivität: Human, Maus, Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human, Mouse and Rat Phosphotyrosine FRS2 ELISA Kit. This assay semi-quantitatively measures tyrosine-phosphorylated FRS2 in cell lysate samples.

Proben: Cell Samples, Tissue Lysate

Analytische Methode: Semi-Quantitative

Spezifität: This ELISA kit recognizes human FRS2 phosphotyrosine.

Produktmerkmale:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Biotinylated Anti-Phosphotyrosine Antibody
• Stop Solution
• Assay Diluent(s)
• Positive Control Sample
• Lysis Buffer
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Biotinylated Anti-Phosphotyrosine Antibody
• Stop Solution
• Assay Diluent(s)
• Positive Control Sample
• Lysis Buffer
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Protokoll:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Haltbarkeit: 6 months

Details zu FRS2

Target: FRS2 (Fibroblast Growth Factor Receptor Substrate 2 (FRS2))

Andere Bezeichnung: FRS2 (FRS2 Produkte)

Synonyme: frs2 ELISA Kit, wu:fd21h10 ELISA Kit, zgc:91973 ELISA Kit, FRS2A ELISA Kit, FRS2alpha ELISA Kit, SNT ELISA Kit, SNT-1 ELISA Kit, SNT1 ELISA Kit, 4732458E18 ELISA Kit, C330018A15Rik ELISA Kit, Frs2alpha ELISA Kit, fibroblast growth factor receptor substrate 2 ELISA Kit, fibroblast growth factor receptor substrate 2b ELISA Kit, FRS2 ELISA Kit, frs2b ELISA Kit, Frs2 ELISA Kit

Gen-ID: 10818

UniProt: Q8WU20, Q8C180

Pathways: Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Signaling Events mediated by VEGFR1 and VEGFR2