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CHD7 ELISA Kit

CHD7 Reaktivität: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Produktnummer ABIN6951098
  • Target Alle CHD7 Produkte
    CHD7 (Chromodomain Helicase DNA Binding Protein 7 (CHD7))
    Reaktivität
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Human CHD7 ELISA Kit.
    Proben
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Haltbarkeit
    6 months
  • Target Alle CHD7 Produkte
    CHD7 (Chromodomain Helicase DNA Binding Protein 7 (CHD7))
    Andere Bezeichnung
    CHD7 (CHD7 Produkte)
    Synonyme
    CHD7 ELISA Kit, fd19h06 ELISA Kit, si:ch211-197o6.2 ELISA Kit, wu:cegs2051 ELISA Kit, wu:fb37f10 ELISA Kit, wu:fb39h04 ELISA Kit, wu:fd19h06 ELISA Kit, HH5 ELISA Kit, IS3 ELISA Kit, KAL5 ELISA Kit, A730019I05Rik ELISA Kit, Cycn ELISA Kit, Cyn ELISA Kit, Dz ELISA Kit, Edy ELISA Kit, Flo ELISA Kit, GENA 47 ELISA Kit, GENA 60 ELISA Kit, Gena 52 ELISA Kit, Lda ELISA Kit, Mt ELISA Kit, Obt ELISA Kit, Todo ELISA Kit, WBE1 ELISA Kit, Whi ELISA Kit, metis ELISA Kit, chromodomain helicase DNA binding protein 7 L homeolog ELISA Kit, chromodomain helicase DNA binding protein 7 ELISA Kit, chd7.L ELISA Kit, CHD7 ELISA Kit, chd7 ELISA Kit, Chd7 ELISA Kit
    Gen-ID
    55636
    UniProt
    Q9P2D1
    Pathways
    Sensory Perception of Sound, Chromatin Binding
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