Cyclin A1 ELISA Kit

Produktnummer ABIN6951110

Produktdetails Cyclin A1 ELISA Kit

Target: Cyclin A1 (CCNA1)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human CCNA1 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Haltbarkeit: 6 months

Details zu Cyclin A1

Target: Cyclin A1 (CCNA1)

Andere Bezeichnung: CCNA1 (CCNA1 Produkte)

Synonyme: fd14f08 ELISA Kit, wu:fd14f08 ELISA Kit, wu:fi20d12 ELISA Kit, zgc:111869 ELISA Kit, MGC81965 ELISA Kit, cyclin-A1 ELISA Kit, CCNA1 ELISA Kit, MGC132235 ELISA Kit, cyclin-A1-2 ELISA Kit, Cyclin-A1 ELISA Kit, ccna1 ELISA Kit, cyclin A1 ELISA Kit, cyclin A1 L homeolog ELISA Kit, CYCLIN A1;2 ELISA Kit, cyclin A1 S homeolog ELISA Kit, CCNA1 ELISA Kit, ccna1 ELISA Kit, ccna1.L ELISA Kit, CYCA1;2 ELISA Kit, Ccna1 ELISA Kit, ccna1.S ELISA Kit

Gen-ID: 8900

UniProt: P78396

Pathways: Apoptose, Zellzyklus, AMPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA