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Ephrin B2 ELISA Kit

EFNB2 Reaktivität: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Produktnummer ABIN6951116
  • Target Alle Ephrin B2 (EFNB2) ELISA Kits anzeigen
    Ephrin B2 (EFNB2)
    Reaktivität
    • 4
    • 3
    • 2
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Human Ephrin-B2 ELISA Kit.
    Proben
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product EFNB2 ELISA Kit
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Haltbarkeit
    6 months
  • Target Alle Ephrin B2 (EFNB2) ELISA Kits anzeigen
    Ephrin B2 (EFNB2)
    Andere Bezeichnung
    Ephrin-B2 (EFNB2 Produkte)
    Synonyme
    EFNB2 ELISA Kit, efnb2 ELISA Kit, ephrin-B2 ELISA Kit, htkl ELISA Kit, eplg5 ELISA Kit, htk-l ELISA Kit, lerk5 ELISA Kit, ephrinB2 ELISA Kit, efnb2a ELISA Kit, MGC68890 ELISA Kit, efnb2b ELISA Kit, ELF-2 ELISA Kit, Epl5 ELISA Kit, Eplg5 ELISA Kit, Htk-L ELISA Kit, LERK-5 ELISA Kit, Lerk5 ELISA Kit, NLERK-1 ELISA Kit, EPLG5 ELISA Kit, HTKL ELISA Kit, LERK5 ELISA Kit, ephrin B2 ELISA Kit, ephrin-B2 precursor ELISA Kit, ephrin B2 L homeolog ELISA Kit, ephrin B2 S homeolog ELISA Kit, EFNB2 ELISA Kit, efnb2 ELISA Kit, efnb2.L ELISA Kit, efnb2.S ELISA Kit, Efnb2 ELISA Kit
    Gen-ID
    1948
    UniProt
    P52799
    Pathways
    RTK Signalweg, Regulation of Muscle Cell Differentiation
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