GCAP1 ELISA Kit

Produktnummer ABIN6951132

Produktdetails GCAP1 ELISA Kit

Target: GCAP1 (GUCA1A) (Guanylate Cyclase Activator 1A (Retina) (GUCA1A))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human GUCA1A ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Haltbarkeit: 6 months

Details zu GCAP1

Target: GCAP1 (GUCA1A) (Guanylate Cyclase Activator 1A (Retina) (GUCA1A))

Andere Bezeichnung: GUCA1A (GUCA1A Produkte)

Synonyme: C6orf131 ELISA Kit, COD3 ELISA Kit, CORD14 ELISA Kit, GCAP ELISA Kit, GCAP1 ELISA Kit, GUCA ELISA Kit, GUCA1 ELISA Kit, dJ139D8.6 ELISA Kit, MGC84170 ELISA Kit, GUCA1A ELISA Kit, guca1a ELISA Kit, MGC146317 ELISA Kit, GC-A ELISA Kit, Gcap1 ELISA Kit, Guca1 ELISA Kit, mGCAP1 ELISA Kit, gcap1 ELISA Kit, guanylate cyclase activator 1A ELISA Kit, guanylate cyclase activator 1A L homeolog ELISA Kit, guanylate cyclase activator 1A (retina) ELISA Kit, guanylate cyclase activator 1a (retina) ELISA Kit, GUCA1A ELISA Kit, Guca1a ELISA Kit, guca1a.L ELISA Kit, guca1a ELISA Kit

Gen-ID: 2978

UniProt: P43080

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction