PRDM2 ELISA Kit

Produktnummer ABIN6951135

Produktdetails PRDM2 ELISA Kit

Target: PRDM2 (PR Domain Containing 2, with ZNF Domain (PRDM2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human PRDM2 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Haltbarkeit: 6 months

Details zu PRDM2

Target: PRDM2 (PR Domain Containing 2, with ZNF Domain (PRDM2))

Andere Bezeichnung: PRDM2 (PRDM2 Produkte)

Synonyme: MGC114763 ELISA Kit, prdm2 ELISA Kit, riz ELISA Kit, kmt8 ELISA Kit, riz1 ELISA Kit, riz2 ELISA Kit, mtb-zf ELISA Kit, humhoxy1 ELISA Kit, PRDM2 ELISA Kit, HUMHOXY1 ELISA Kit, KMT8 ELISA Kit, MTB-ZF ELISA Kit, RIZ ELISA Kit, RIZ1 ELISA Kit, RIZ2 ELISA Kit, 4833427P12Rik ELISA Kit, E330024L24Rik ELISA Kit, Gm1033 ELISA Kit, Gm19732 ELISA Kit, Riz ELISA Kit, Riz1 ELISA Kit, Znfpr1c1 ELISA Kit, PR domain containing 2, with ZNF domain S homeolog ELISA Kit, PR domain containing 2, with ZNF domain b ELISA Kit, PR domain containing 2, with ZNF domain ELISA Kit, PR domain zinc finger protein 2 ELISA Kit, PR/SET domain 2 ELISA Kit, prdm2.S ELISA Kit, prdm2b ELISA Kit, prdm2 ELISA Kit, LOC100350859 ELISA Kit, PRDM2 ELISA Kit, Prdm2 ELISA Kit

Gen-ID: 7799

UniProt: Q13029