GGPS1 ELISA Kit

Produktnummer ABIN6951136

Produktdetails GGPS1 ELISA Kit

Target: GGPS1 (Geranylgeranyl Diphosphate Synthase 1 (GGPS1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human GGPS1 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Haltbarkeit: 6 months

Details zu GGPS1

Target: GGPS1 (Geranylgeranyl Diphosphate Synthase 1 (GGPS1))

Andere Bezeichnung: GGPS1 (GGPS1 Produkte)

Synonyme: geranylgeranyl pyrophosphate synthase 1 ELISA Kit, DDBDRAFT_0192027 ELISA Kit, DDBDRAFT_0233098 ELISA Kit, DDB_0192027 ELISA Kit, DDB_0233098 ELISA Kit, GGPPS ELISA Kit, GGPPS1 ELISA Kit, 1810026C22Rik ELISA Kit, 9530089B04Rik ELISA Kit, AI843169 ELISA Kit, C79210 ELISA Kit, Crlf3 ELISA Kit, rGGPS1a ELISA Kit, rGGPS1a1 ELISA Kit, rGGPS1a2 ELISA Kit, rGGPS1a3 ELISA Kit, fb05f09 ELISA Kit, qm ELISA Kit, wu:fb05f09 ELISA Kit, zgc:101591 ELISA Kit, zgc:56514 ELISA Kit, geranylgeranyl pyrophosphate synthase 1 ELISA Kit, geranylgeranyl pyrophosphate synthetase ELISA Kit, geranyl pyrophosphate synthase ELISA Kit, geranylgeranyl pyrophosphate synthase,chloroplastic ELISA Kit, geranylgeranyl diphosphate synthase 1 ELISA Kit, geranylgeranyl diphosphate synthase 1 L homeolog ELISA Kit, GGPS1 ELISA Kit, gGPS1 ELISA Kit, ggps1 ELISA Kit, Ggps1 ELISA Kit, ggps1.L ELISA Kit

Gen-ID: 9453

UniProt: O95749