ING5 ELISA Kit

Produktnummer ABIN6951148

Produktdetails ING5 ELISA Kit

Target: ING5 (Inhibitor of Growth Family, Member 5 (ING5))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human ING5 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Haltbarkeit: 6 months

Details zu ING5

Target: ING5 (Inhibitor of Growth Family, Member 5 (ING5))

Andere Bezeichnung: ING5 (ING5 Produkte)

Synonyme: p28ING5 ELISA Kit, 1700001C14Rik ELISA Kit, 1700027H23Rik ELISA Kit, 1810018M11Rik ELISA Kit, AI225768 ELISA Kit, p28ing5 ELISA Kit, MGC76007 ELISA Kit, ING5 ELISA Kit, MGC133862 ELISA Kit, ING1 ELISA Kit, p33 ELISA Kit, si:busm1-40g1.1 ELISA Kit, si:dz40g1.1 ELISA Kit, wu:fc94a02 ELISA Kit, zgc:100910 ELISA Kit, ing5 ELISA Kit, inhibitor of growth family member 5 ELISA Kit, inhibitor of growth family, member 5 ELISA Kit, inhibitor of growth family member 5 L homeolog ELISA Kit, inhibitor of growth family, member 5b ELISA Kit, inhibitor of growth family, member 5a ELISA Kit, ING5 ELISA Kit, Ing5 ELISA Kit, ing5 ELISA Kit, ing5.L ELISA Kit, ing5b ELISA Kit, ing5a ELISA Kit

Gen-ID: 84289

UniProt: Q8WYH8

Pathways: Chromatin Binding