PDZD11 ELISA Kit

Produktnummer ABIN6951209

Produktdetails PDZD11 ELISA Kit

Target: PDZD11 (PDZ Domain Containing 11 (PDZD11))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human PDZD11 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Haltbarkeit: 6 months

Details zu PDZD11

Target: PDZD11 (PDZ Domain Containing 11 (PDZD11))

Andere Bezeichnung: PDZD11 (PDZD11 Produkte)

Synonyme: RGD1560007 ELISA Kit, MGC89018 ELISA Kit, AIPP1 ELISA Kit, PDZK11 ELISA Kit, PISP ELISA Kit, 1810012H22Rik ELISA Kit, 2310079D11Rik ELISA Kit, 2700099C19Rik ELISA Kit, AI854765 ELISA Kit, Pdzk11 ELISA Kit, zgc:77536 ELISA Kit, PDZ domain containing 11 ELISA Kit, PDZ domain containing 11 L homeolog ELISA Kit, Pdzd11 ELISA Kit, pdzd11.L ELISA Kit, pdzd11 ELISA Kit, PDZD11 ELISA Kit

Gen-ID: 51248

UniProt: Q5EBL8

Pathways: SARS-CoV-2 Protein Interaktom