PMVK ELISA Kit

Produktnummer ABIN6951213

Produktdetails PMVK ELISA Kit

Target: PMVK (Phosphomevalonate Kinase (PMVK))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human PMVK ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Haltbarkeit: 6 months

Details zu PMVK

Target: PMVK (Phosphomevalonate Kinase (PMVK))

Andere Bezeichnung: PMVK (PMVK Produkte)

Synonyme: MPK_Bm ELISA Kit, F5M6.9 ELISA Kit, F5M6_9 ELISA Kit, zgc:162701 ELISA Kit, HUMPMKI ELISA Kit, PMK ELISA Kit, PMKA ELISA Kit, PMKASE ELISA Kit, 1110011E12Rik ELISA Kit, 2900002L22Rik ELISA Kit, AV001327 ELISA Kit, phosphomevalonate kinase ELISA Kit, GHMP kinase family protein ELISA Kit, PMVK ELISA Kit, Mpk ELISA Kit, AT1G31910 ELISA Kit, CpipJ_CPIJ015413 ELISA Kit, Bm1_05275 ELISA Kit, pmvk ELISA Kit, LOAG_11811 ELISA Kit, Tsp_06629 ELISA Kit, LOC100282968 ELISA Kit, Pmvk ELISA Kit

Gen-ID: 10654

UniProt: Q15126