PSPH ELISA Kit

Produktnummer ABIN6951215

Produktdetails PSPH ELISA Kit

Target: PSPH (Phosphoserine Phosphatase (PSPH))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human PSPH ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Haltbarkeit: 6 months

Details zu PSPH

Target: PSPH (Phosphoserine Phosphatase (PSPH))

Andere Bezeichnung: PSPH (PSPH Produkte)

Synonyme: PSP ELISA Kit, PSPHD ELISA Kit, AI480570 ELISA Kit, PSPase ELISA Kit, PSPH ELISA Kit, psph ELISA Kit, MGC78828 ELISA Kit, zgc:112414 ELISA Kit, DDBDRAFT_0183847 ELISA Kit, DDBDRAFT_0230054 ELISA Kit, DDB_0183847 ELISA Kit, DDB_0230054 ELISA Kit, phosphoserine phosphatase ELISA Kit, phosphoserine phosphatase L homeolog ELISA Kit, PSP; O-phosphoserine phosphohydrolase; PSPase ELISA Kit, phosphoserine phosphatase SerB ELISA Kit, BPI fold containing family A member 2 ELISA Kit, PSPH ELISA Kit, Psph ELISA Kit, psph.L ELISA Kit, psph ELISA Kit, serB ELISA Kit, serB-1 ELISA Kit, LOC5568588 ELISA Kit, PSP ELISA Kit, Svir_29300 ELISA Kit, MMAH_RS03435 ELISA Kit, BPIFA2 ELISA Kit

Gen-ID: 5723

UniProt: P78330

Pathways: Warburg Effekt