Syntaxin 7 ELISA Kit

Produktnummer ABIN6951239

Produktdetails Syntaxin 7 ELISA Kit

Target: Syntaxin 7 (STX7)

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Rat STX7 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Haltbarkeit: 6 months

Details zu Syntaxin 7

Target: Syntaxin 7 (STX7)

Andere Bezeichnung: STX7 (STX7 Produkte)

Synonyme: Aval ELISA Kit, Avl ELISA Kit, CG5081 ELISA Kit, Dm Syx7 ELISA Kit, DmSyx7 ELISA Kit, Dmel\\CG5081 ELISA Kit, FCP-C ELISA Kit, SYX7 ELISA Kit, Syx ELISA Kit, avl ELISA Kit, dSyn7 ELISA Kit, syx7 ELISA Kit, syx7/avl ELISA Kit, syntaxin-7 ELISA Kit, F27G20.2 ELISA Kit, SYNTAXIN 24 ELISA Kit, SYP24 ELISA Kit, DDBDRAFT_0187598 ELISA Kit, DDBDRAFT_0231537 ELISA Kit, DDB_0187598 ELISA Kit, DDB_0231537 ELISA Kit, Syntaxin-7 ELISA Kit, STX7 ELISA Kit, AI315064 ELISA Kit, AI317144 ELISA Kit, AW107239 ELISA Kit, Syn7 ELISA Kit, Syntaxin 7 ELISA Kit, syntaxin 7 ELISA Kit, syntaxin ELISA Kit, Syx7 ELISA Kit, STX7 ELISA Kit, ATSYP24 ELISA Kit, syn7A ELISA Kit, stx7 ELISA Kit, Stx7 ELISA Kit

Gen-ID: 60466

UniProt: O70257

Pathways: Synaptic Vesicle Exocytosis