COX5B ELISA Kit
COX5B
Reaktivität: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Produktnummer ABIN6951254
-
-
Target
Alle COX5B Produkte
-
COX5B
(Cytochrome C Oxidase Subunit Vb (COX5B))
-
Reaktivität
-
Human
-
Nachweismethode
-
Colorimetric
-
Methodentyp
-
Sandwich ELISA
-
Applikation
-
ELISA
-
Verwendungszweck
-
Human COX5B ELISA Kit.
-
Proben
-
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
-
Analytische Methode
-
Quantitative
-
Produktmerkmale
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
-
Bestandteile
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
-
Benötigtes Material
-
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
-
-
Applikationshinweise
-
Optimal working dilution should be determined by the investigator.
-
Plattentyp
-
Pre-coated
-
Protokoll
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
-
Beschränkungen
-
Nur für Forschungszwecke einsetzbar
-
-
Haltbarkeit
-
6 months
-
-
Target
Alle COX5B Produkte
-
COX5B
(Cytochrome C Oxidase Subunit Vb (COX5B))
-
Andere Bezeichnung
-
COX5B (COX5B Produkte)
-
Synonyme
-
COXVB ELISA Kit, coxvb ELISA Kit, cox5b ELISA Kit, GB16568 ELISA Kit, 17.m07945 ELISA Kit, COX5B ELISA Kit, CoVb ELISA Kit, Dsim\\GD23419 ELISA Kit, GD23419 ELISA Kit, dsim_GLEANR_7156 ELISA Kit, cytochrome c oxidase subunit 5B ELISA Kit, cytochrome c oxidase subunit Vb ELISA Kit, cytochrome c oxidase subunit Vb, gene 2 L homeolog ELISA Kit, cytochrome c oxidase subunit Vb, gene 1 ELISA Kit, cytochrome c oxidase subunit 5B, mitochondrial ELISA Kit, mitochondrial cytochrome c oxidase subunit Vb ELISA Kit, COX5B ELISA Kit, Cox5b ELISA Kit, cox5b.2.L ELISA Kit, cox5b.1 ELISA Kit, LOC552610 ELISA Kit, BBOV_III010970 ELISA Kit, Dsim\CoVb ELISA Kit
-
Gen-ID
-
1329
-
UniProt
-
P10606
-