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COX5B ELISA Kit

COX5B Reaktivität: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Produktnummer ABIN6951254
  • Target Alle COX5B Produkte
    COX5B (Cytochrome C Oxidase Subunit Vb (COX5B))
    Reaktivität
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Human COX5B ELISA Kit.
    Proben
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Haltbarkeit
    6 months
  • Target Alle COX5B Produkte
    COX5B (Cytochrome C Oxidase Subunit Vb (COX5B))
    Andere Bezeichnung
    COX5B (COX5B Produkte)
    Synonyme
    COXVB ELISA Kit, coxvb ELISA Kit, cox5b ELISA Kit, GB16568 ELISA Kit, 17.m07945 ELISA Kit, COX5B ELISA Kit, CoVb ELISA Kit, Dsim\\GD23419 ELISA Kit, GD23419 ELISA Kit, dsim_GLEANR_7156 ELISA Kit, cytochrome c oxidase subunit 5B ELISA Kit, cytochrome c oxidase subunit Vb ELISA Kit, cytochrome c oxidase subunit Vb, gene 2 L homeolog ELISA Kit, cytochrome c oxidase subunit Vb, gene 1 ELISA Kit, cytochrome c oxidase subunit 5B, mitochondrial ELISA Kit, mitochondrial cytochrome c oxidase subunit Vb ELISA Kit, COX5B ELISA Kit, Cox5b ELISA Kit, cox5b.2.L ELISA Kit, cox5b.1 ELISA Kit, LOC552610 ELISA Kit, BBOV_III010970 ELISA Kit, Dsim\CoVb ELISA Kit
    Gen-ID
    1329
    UniProt
    P10606
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