FDPS ELISA Kit

Produktnummer ABIN6951338

Produktdetails FDPS ELISA Kit

Target: FDPS (Farnesyl Diphosphate Synthase (FDPS))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human FDPS ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Haltbarkeit: 6 months

Details zu FDPS

Target: FDPS (Farnesyl Diphosphate Synthase (FDPS))

Andere Bezeichnung: FDPS (FDPS Produkte)

Synonyme: CG12389 ELISA Kit, DmFPPS ELISA Kit, DmFPPSase ELISA Kit, DmFPS ELISA Kit, Dmel\\CG12389 ELISA Kit, FPS ELISA Kit, fpps ELISA Kit, fps ELISA Kit, l(2)k06103 ELISA Kit, v(2)k03514 ELISA Kit, NCU01175.1 ELISA Kit, 16.m00760 ELISA Kit, DDBDRAFT_0206220 ELISA Kit, DDBDRAFT_0215017 ELISA Kit, DDB_0206220 ELISA Kit, DDB_0215017 ELISA Kit, FPPS ELISA Kit, Ac2-125 ELISA Kit, (2E ELISA Kit, 6030492I17Rik ELISA Kit, AI256750 ELISA Kit, Fdpsl1 ELISA Kit, mKIAA1293 ELISA Kit, Farnesyl pyrophosphate synthase ELISA Kit, farnesyl diphosphate synthase ELISA Kit, farnesyl diphosphate synthase L homeolog ELISA Kit, farnesyl pyrophosphate synthase ELISA Kit, farnesyl pyrophosphate synthetase ELISA Kit, farnesyl-pyrophosphate synthetase ELISA Kit, FDP synthase ELISA Kit, Farnesyl pyrophosphate synthetase ELISA Kit, farnesyl diphosphate synthetase ELISA Kit, farnesyl pyrophosphate synthase 1 ELISA Kit, Fpps ELISA Kit, fdps ELISA Kit, fdps.L ELISA Kit, MGG_08649 ELISA Kit, TP03_0857 ELISA Kit, Tb09.160.4300 ELISA Kit, fpp ELISA Kit, BBOV_I000120 ELISA Kit, fps ELISA Kit, fpps ELISA Kit, FDPS ELISA Kit, Fdps ELISA Kit, fps1 ELISA Kit

Gen-ID: 2224

UniProt: P14324

Pathways: Regulation of Muscle Cell Differentiation