PEBP1 ELISA Kit

Produktnummer ABIN6951355

Produktdetails PEBP1 ELISA Kit

Target: PEBP1 (Phosphatidylethanolamine Binding Protein 1 (PEBP1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human PEBP1 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Haltbarkeit: 6 months

Details zu PEBP1

Target: PEBP1 (Phosphatidylethanolamine Binding Protein 1 (PEBP1))

Andere Bezeichnung: PEBP1 (PEBP1 Produkte)

Synonyme: HCNP ELISA Kit, HCNPpp ELISA Kit, PBP ELISA Kit, PEBP ELISA Kit, PEBP-1 ELISA Kit, RKIP ELISA Kit, pbp ELISA Kit, hcnp ELISA Kit, pebp ELISA Kit, rkip ELISA Kit, Pbp ELISA Kit, Pbp1 ELISA Kit, Pbpr ELISA Kit, Rkip ELISA Kit, PEBP1 ELISA Kit, BcDNA:LP12095 ELISA Kit, CG18594 ELISA Kit, Dmel\\CG18594 ELISA Kit, T2 ELISA Kit, zgc:56033 ELISA Kit, zgc:76942 ELISA Kit, phosphatidylethanolamine binding protein 1 ELISA Kit, phosphatidylethanolamine binding protein 1 L homeolog ELISA Kit, phosphatidylethanolamine-binding protein ELISA Kit, phosphatidylethanolamine-binding protein,putative ELISA Kit, Phosphatidylethanolamine-binding protein 1 ELISA Kit, PEBP1 ELISA Kit, pebp1 ELISA Kit, Pebp1 ELISA Kit, pebp1.L ELISA Kit, RR_RS07785 ELISA Kit, PVX_235290 ELISA Kit, PVX_123630 ELISA Kit, PKH_143640 ELISA Kit, EAM_1207 ELISA Kit

Gen-ID: 5037

UniProt: P30086

Pathways: Feeding Behaviour