EIF5A2 ELISA Kit

Produktnummer ABIN6951365

Produktdetails EIF5A2 ELISA Kit

Target: EIF5A2 (Eukaryotic Translation Initiation Factor 5A2 (EIF5A2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human EIF5A2 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Haltbarkeit: 6 months

Details zu EIF5A2

Target: EIF5A2 (Eukaryotic Translation Initiation Factor 5A2 (EIF5A2))

Andere Bezeichnung: EIF5A2 (EIF5A2 Produkte)

Synonyme: EIF5A2 ELISA Kit, EIF5A ELISA Kit, zgc:55504 ELISA Kit, zgc:77429 ELISA Kit, wu:fb05b06 ELISA Kit, wu:fc23f04 ELISA Kit, wu:fc61c10 ELISA Kit, EIF-5A2 ELISA Kit, eIF5AII ELISA Kit, 9630038B20 ELISA Kit, eukaryotic translation initiation factor 5A2 ELISA Kit, eukaryotic translation initiation factor 5A3 ELISA Kit, EIF5A2 ELISA Kit, eif5a2 ELISA Kit, LOC100499632 ELISA Kit, Eif5a2 ELISA Kit

Gen-ID: 56648

UniProt: Q9GZV4