NT5C3 ELISA Kit
NT5C3
Reaktivität: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Produktnummer ABIN6951367
-
-
Target
Alle NT5C3 Produkte
-
NT5C3
(5'-Nucleotidase, Cytosolic III (NT5C3))
-
Reaktivität
-
Human
-
Nachweismethode
-
Colorimetric
-
Methodentyp
-
Sandwich ELISA
-
Applikation
-
ELISA
-
Verwendungszweck
-
Human NT5C3A ELISA Kit.
-
Proben
-
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
-
Analytische Methode
-
Quantitative
-
Produktmerkmale
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
-
Bestandteile
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
-
Benötigtes Material
-
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
-
-
Applikationshinweise
-
Optimal working dilution should be determined by the investigator.
-
Plattentyp
-
Pre-coated
-
Protokoll
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
-
Beschränkungen
-
Nur für Forschungszwecke einsetzbar
-
-
Haltbarkeit
-
6 months
-
-
Target
Alle NT5C3 Produkte
-
NT5C3
(5'-Nucleotidase, Cytosolic III (NT5C3))
-
Andere Bezeichnung
-
NT5C3A (NT5C3 Produkte)
-
Synonyme
-
1600024P05Rik ELISA Kit, 2610206B05Rik ELISA Kit, 3110004A18Rik ELISA Kit, AI842776 ELISA Kit, Nt5c3a ELISA Kit, Nt5y ELISA Kit, PN-1 ELISA Kit, PN-I ELISA Kit, PSN1 ELISA Kit, UMPH ELISA Kit, Umph-1 ELISA Kit, Umph1 ELISA Kit, cN-III ELISA Kit, lupin ELISA Kit, p36 ELISA Kit, fb30h07 ELISA Kit, nt5c3 ELISA Kit, wu:fb30h07 ELISA Kit, zgc:66117 ELISA Kit, NT5C3 ELISA Kit, PYN1 ELISA Kit, P5'N-1 ELISA Kit, P5N-1 ELISA Kit, POMP ELISA Kit, UMPH1 ELISA Kit, hUMP1 ELISA Kit, Nt5c3 ELISA Kit, 5'-nucleotidase, cytosolic III ELISA Kit, 5'-nucleotidase, cytosolic IIIA ELISA Kit, cytosolic 5'-nucleotidase 3A ELISA Kit, Nt5c3 ELISA Kit, nt5c3a ELISA Kit, NT5C3A ELISA Kit, LOC534181 ELISA Kit, Nt5c3a ELISA Kit, nt5c3a.L ELISA Kit, Bm1_56095 ELISA Kit
-
Gen-ID
-
51251
-
UniProt
-
Q9H0P0
-