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CRIP2 ELISA Kit

CRIP2 Reaktivität: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Produktnummer ABIN6951411
  • Target Alle CRIP2 Produkte
    CRIP2 (Cysteine-Rich Protein 2 (CRIP2))
    Reaktivität
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Human CRIP2 ELISA Kit.
    Proben
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Haltbarkeit
    6 months
  • Target Alle CRIP2 Produkte
    CRIP2 (Cysteine-Rich Protein 2 (CRIP2))
    Andere Bezeichnung
    CRIP2 (CRIP2 Produkte)
    Synonyme
    CRIP ELISA Kit, CRP2 ELISA Kit, ESP1 ELISA Kit, C6orf210 ELISA Kit, COQ10D3 ELISA Kit, DLP1 ELISA Kit, bA59I9.3 ELISA Kit, hDLP1 ELISA Kit, AW551867 ELISA Kit, Crp2 ELISA Kit, SmLim ELISA Kit, SmLIM ELISA Kit, zgc:77740 ELISA Kit, 0610010I23Rik ELISA Kit, AW743261 ELISA Kit, C77570 ELISA Kit, CRP4 ELISA Kit, Crp ELISA Kit, Hlp ELISA Kit, wu:fb30h10 ELISA Kit, zgc:55408 ELISA Kit, cysteine rich protein 2 ELISA Kit, decaprenyl diphosphate synthase subunit 2 ELISA Kit, cysteine and glycine-rich protein 2 ELISA Kit, cysteine and glycine rich protein 2 ELISA Kit, cysteine-rich protein 2 ELISA Kit, CRIP2 ELISA Kit, PDSS2 ELISA Kit, Csrp2 ELISA Kit, csrp2 ELISA Kit, CSRP2 ELISA Kit, Crip2 ELISA Kit, crip2 ELISA Kit
    Gen-ID
    1397
    UniProt
    P52943
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