DREAM ELISA Kit

Produktnummer ABIN6951435

Produktdetails DREAM ELISA Kit

Target: DREAM (KCNIP3) (Kv Channel Interacting Protein 3, Calsenilin (KCNIP3))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human KCNIP3 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Haltbarkeit: 6 months

Details zu DREAM

Target: DREAM (KCNIP3) (Kv Channel Interacting Protein 3, Calsenilin (KCNIP3))

Andere Bezeichnung: KCNIP3 (KCNIP3 Produkte)

Synonyme: CSEN ELISA Kit, DREAM ELISA Kit, KCHIP3 ELISA Kit, 4933407H12Rik ELISA Kit, AI413860 ELISA Kit, Csen ELISA Kit, KChIP3 ELISA Kit, R74849 ELISA Kit, Dream ELISA Kit, rKChIP3 ELISA Kit, csen ELISA Kit, im:7145308 ELISA Kit, kcnip3 ELISA Kit, zgc:73306 ELISA Kit, KCNIP3 ELISA Kit, potassium voltage-gated channel interacting protein 3 ELISA Kit, Kv channel-interacting protein 4 ELISA Kit, calsenilin ELISA Kit, Kv channel interacting protein 3, calsenilin ELISA Kit, Kv channel interacting protein 3b, calsenilin ELISA Kit, KCNIP3 ELISA Kit, LOC5568664 ELISA Kit, CpipJ_CPIJ002549 ELISA Kit, Tsp_05009 ELISA Kit, Kcnip3 ELISA Kit, kcnip3b ELISA Kit, kcnip3 ELISA Kit

Gen-ID: 30818

UniProt: Q9Y2W7