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NDRG1 ELISA Kit

NDRG1 Reaktivität: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Produktnummer ABIN6951436
  • Target Alle NDRG1 ELISA Kits anzeigen
    NDRG1 (N-Myc Downstream Regulated 1 (NDRG1))
    Reaktivität
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Human NDRG1 ELISA Kit.
    Proben
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Haltbarkeit
    6 months
  • Target Alle NDRG1 ELISA Kits anzeigen
    NDRG1 (N-Myc Downstream Regulated 1 (NDRG1))
    Andere Bezeichnung
    NDRG1 (NDRG1 Produkte)
    Synonyme
    CAP43 ELISA Kit, CMT4D ELISA Kit, DRG-1 ELISA Kit, DRG1 ELISA Kit, GC4 ELISA Kit, HMSNL ELISA Kit, NDR1 ELISA Kit, NMSL ELISA Kit, PROXY1 ELISA Kit, RIT42 ELISA Kit, RTP ELISA Kit, TARG1 ELISA Kit, TDD5 ELISA Kit, Ndr1 ELISA Kit, Ndrl ELISA Kit, cap43 ELISA Kit, cmt4d ELISA Kit, drg1 ELISA Kit, gc4 ELISA Kit, hmsnl ELISA Kit, ndr1 ELISA Kit, ndrg1 ELISA Kit, nmsl ELISA Kit, proxy1 ELISA Kit, rit42 ELISA Kit, rtp ELISA Kit, targ1 ELISA Kit, tdd5 ELISA Kit, xndrg1 ELISA Kit, ndrg4 ELISA Kit, cb775 ELISA Kit, wu:fb60h02 ELISA Kit, zgc:63944 ELISA Kit, NDRG1 ELISA Kit, NON-RACE SPECIFIC DISEASE RESISTANCE PROTEIN ELISA Kit, non race-specific disease resistance 1 ELISA Kit, ndrg1-a ELISA Kit, ndrg1-b ELISA Kit, xNDRG1-A ELISA Kit, ndrg1l ELISA Kit, zgc:73047 ELISA Kit, N-myc downstream regulated 1 ELISA Kit, N-myc downstream regulated gene 1 ELISA Kit, N-myc downstream regulated 1 S homeolog ELISA Kit, N-myc downstream regulated 1a ELISA Kit, Late embryogenesis abundant (LEA) hydroxyproline-rich glycoprotein family ELISA Kit, N-myc downstream regulated 1 L homeolog ELISA Kit, N-myc downstream regulated 1b ELISA Kit, NDRG1 ELISA Kit, Ndrg1 ELISA Kit, ndrg1.S ELISA Kit, ndrg1 ELISA Kit, ndrg1a ELISA Kit, NDR1 ELISA Kit, ndrg1.L ELISA Kit, ndrg1b ELISA Kit
    Gen-ID
    10397
    UniProt
    Q92597
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