DHRS9 ELISA Kit

Produktnummer ABIN6951437

Produktdetails DHRS9 ELISA Kit

Target: DHRS9 (Dehydrogenase/reductase (SDR Family) Member 9 (DHRS9))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human DHRS9 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Haltbarkeit: 6 months

Details zu DHRS9

Target: DHRS9 (Dehydrogenase/reductase (SDR Family) Member 9 (DHRS9))

Andere Bezeichnung: DHRS9 (DHRS9 Produkte)

Synonyme: 3ALPHA-HSD ELISA Kit, RDH-TBE ELISA Kit, RDH15 ELISA Kit, RDHL ELISA Kit, RDHTBE ELISA Kit, RETSDR8 ELISA Kit, SDR9C4 ELISA Kit, Rdhl ELISA Kit, C730025I08Rik ELISA Kit, Rdh15 ELISA Kit, rdhl ELISA Kit, 3alpha-hsd ELISA Kit, rdh15 ELISA Kit, retsdr8 ELISA Kit, DHRS9 ELISA Kit, RDHA ELISA Kit, rdh1l ELISA Kit, wu:fb64b07 ELISA Kit, zgc:73286 ELISA Kit, dehydrogenase/reductase 9 ELISA Kit, dehydrogenase/reductase (SDR family) member 9 ELISA Kit, dehydrogenase/reductase (SDR family) member 9 L homeolog ELISA Kit, DHRS9 ELISA Kit, Dhrs9 ELISA Kit, dhrs9.L ELISA Kit, dhrs9 ELISA Kit

Gen-ID: 10170

UniProt: Q9BPW9

Pathways: C21-Steroid Hormone Metabolic Process