FYN ELISA Kit

Produktnummer ABIN6951438

Produktdetails FYN ELISA Kit

Target: FYN (FYN Oncogene Related To SRC, FGR, YES (FYN))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human Fyn ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Haltbarkeit: 6 months

Details zu FYN

Target: FYN (FYN Oncogene Related To SRC, FGR, YES (FYN))

Andere Bezeichnung: Fyn (FYN Produkte)

Synonyme: im:6908851 ELISA Kit, si:dkey-119m7.5 ELISA Kit, zgc:109996 ELISA Kit, c-fyn ELISA Kit, cfyn-a ELISA Kit, SLK ELISA Kit, SYN ELISA Kit, p59-FYN ELISA Kit, PKC ELISA Kit, fyn ELISA Kit, zgc:86720 ELISA Kit, AI448320 ELISA Kit, AW552119 ELISA Kit, FYN proto-oncogene, Src family tyrosine kinase ELISA Kit, FYN proto-oncogene, Src family tyrosine kinase S homeolog ELISA Kit, FYN proto-oncogene, Src family tyrosine kinase b ELISA Kit, FYN proto-oncogene, Src family tyrosine kinase L homeolog ELISA Kit, FYN proto-oncogene, Src family tyrosine kinase a ELISA Kit, Fyn proto-oncogene ELISA Kit, FYN ELISA Kit, fyn.S ELISA Kit, fynb ELISA Kit, fyn.L ELISA Kit, Fyn ELISA Kit, fyna ELISA Kit

Gen-ID: 2534

UniProt: P06241

Pathways: JAK-STAT Signalweg, T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Feeding Behaviour, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Activated T Cell Proliferation, Thromboxane A2 Receptor Signaling