Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

PPT1 ELISA Kit

PPT1 Reaktivität: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Produktnummer ABIN6951472
  • Target Alle PPT1 ELISA Kits anzeigen
    PPT1 (Palmitoyl-Protein Thioesterase 1 (PPT1))
    Reaktivität
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Human PPT1 ELISA Kit.
    Proben
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Haltbarkeit
    6 months
  • Target Alle PPT1 ELISA Kits anzeigen
    PPT1 (Palmitoyl-Protein Thioesterase 1 (PPT1))
    Andere Bezeichnung
    PPT1 (PPT1 Produkte)
    Synonyme
    CLN1 ELISA Kit, INCL ELISA Kit, PPT ELISA Kit, 9530043G02Rik ELISA Kit, AA960502 ELISA Kit, C77813 ELISA Kit, D4Ertd184e ELISA Kit, Ppt ELISA Kit, wu:fj17f04 ELISA Kit, zgc:63969 ELISA Kit, PPT-1 ELISA Kit, ppt1 ELISA Kit, palmitoyl-protein thioesterase 1 ELISA Kit, Palmitoyl-protein thioesterase 1 ELISA Kit, palmitoyl-protein thioesterase 1 (ceroid-lipofuscinosis, neuronal 1, infantile) ELISA Kit, palmitoyl-protein thioesterase 1 L homeolog ELISA Kit, PPT1 ELISA Kit, MGYG_00692 ELISA Kit, Ppt1 ELISA Kit, ppt-1 ELISA Kit, ppt1 ELISA Kit, ppt1.L ELISA Kit
    Gen-ID
    5538
    UniProt
    P50897
    Pathways
    SARS-CoV-2 Protein Interaktom
Sie sind hier:
Kundenservice