Cytidine Monophosphate (UMP-CMP) Kinase 1, Cytosolic (CMPK1) ELISA Kit

Produktnummer ABIN6951480

Produktdetails

Target: Cytidine Monophosphate (UMP-CMP) Kinase 1, Cytosolic (CMPK1)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human CMPK1 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Haltbarkeit: 6 months

Antigendetails

Target: Cytidine Monophosphate (UMP-CMP) Kinase 1, Cytosolic (CMPK1)

Andere Bezeichnung: CMPK1 (CMPK1 Produkte)

Synonyme: CK ELISA Kit, CMK ELISA Kit, CMPK ELISA Kit, UMK ELISA Kit, UMP-CMPK ELISA Kit, UMPK ELISA Kit, UMP/CMPK ELISA Kit, PSPTO1749 ELISA Kit, cmpk1 ELISA Kit, fb52d08 ELISA Kit, fk47a07 ELISA Kit, ump ELISA Kit, wu:fb52d08 ELISA Kit, wu:fk47a07 ELISA Kit, zgc:56452 ELISA Kit, Cmpk ELISA Kit, RGD1310116 ELISA Kit, cmpk ELISA Kit, 0610011D08Rik ELISA Kit, UMP-CMPk ELISA Kit, UCK ELISA Kit, cytidine/uridine monophosphate kinase 1 ELISA Kit, cytidylate kinase ELISA Kit, cytidine monophosphate (UMP-CMP) kinase 1, cytosolic ELISA Kit, cytidine monophosphate (UMP-CMP) kinase 1, cytosolic S homeolog ELISA Kit, cytidylate kinase Cmk ELISA Kit, cytidine monophosphate (UMP-CMP) kinase 1 ELISA Kit, CMPK1 ELISA Kit, cmk ELISA Kit, cmpk1 ELISA Kit, cmpk ELISA Kit, Cmpk1 ELISA Kit, cmpk1.S ELISA Kit, Mrub_0478 ELISA Kit, MMAH_RS06670 ELISA Kit, TAGG_RS04320 ELISA Kit, Trad_0784 ELISA Kit, Deba_2713 ELISA Kit, Toce_1154 ELISA Kit, VDIS_RS03585 ELISA Kit, Fbal_1939 ELISA Kit, Ilyop_1379 ELISA Kit, Lbys_3481 ELISA Kit, Calni_0093 ELISA Kit, Ftrac_1556 ELISA Kit, Ocepr_1777 ELISA Kit

Gen-ID: 51727

UniProt: P30085

Pathways: Nucleotide Phosphorylation, Ribonucleoside Biosynthetic Process