m1ip1 ELISA Kit

Produktnummer ABIN6951500

Produktdetails m1ip1 ELISA Kit

Target: m1ip1 (Mid1-Interacting Protein 1 (m1ip1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human MID1IP1 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Haltbarkeit: 6 months

Details zu m1ip1

Target: m1ip1 (Mid1-Interacting Protein 1 (m1ip1))

Andere Bezeichnung: MID1IP1 (m1ip1 Produkte)

Synonyme: MIG12 ELISA Kit, TB10 ELISA Kit, G12-like ELISA Kit, S14R ELISA Kit, STRAIT11499 ELISA Kit, THRSPL ELISA Kit, 3110038L01Rik ELISA Kit, Mig12 ELISA Kit, slap ELISA Kit, mig12 ELISA Kit, thrspl ELISA Kit, MGC80850 ELISA Kit, zgc:73223 ELISA Kit, g12 ELISA Kit, wu:fj32f05 ELISA Kit, thymosin beta 10 ELISA Kit, MID1 interacting protein 1 ELISA Kit, Mid1-interacting protein 1 ELISA Kit, Mid1 interacting protein 1 (gastrulation specific G12-like (zebrafish)) ELISA Kit, MID1 interacting protein 1 S homeolog ELISA Kit, MID1 interacting protein 1b ELISA Kit, MID1 interacting protein 1a ELISA Kit, TMSB10 ELISA Kit, MID1IP1 ELISA Kit, m1ip1 ELISA Kit, Mid1ip1 ELISA Kit, mid1ip1.S ELISA Kit, mid1ip1b ELISA Kit, mid1ip1a ELISA Kit

Gen-ID: 58526

UniProt: Q9NPA3