ACBD6 ELISA Kit

Produktnummer ABIN6951513

Produktdetails ACBD6 ELISA Kit

Target: ACBD6 (Acyl-CoA Binding Domain Containing 6 (ACBD6))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human ACBD6 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Haltbarkeit: 6 months

Details zu ACBD6

Target: ACBD6 (Acyl-CoA Binding Domain Containing 6 (ACBD6))

Andere Bezeichnung: ACBD6 (ACBD6 Produkte)

Synonyme: MGC79661 ELISA Kit, DDBDRAFT_0188797 ELISA Kit, DDBDRAFT_0266382 ELISA Kit, DDB_0188797 ELISA Kit, DDB_0266382 ELISA Kit, ACBP ELISA Kit, ACYL-COA-BINDING PROTEIN ELISA Kit, F5M6.27 ELISA Kit, F5M6_27 ELISA Kit, acyl-CoA-binding protein 6 ELISA Kit, 0610010G04Rik ELISA Kit, 2610100E10Rik ELISA Kit, wu:fc18d09 ELISA Kit, wu:fc69g03 ELISA Kit, LHX4 antisense RNA 1 ELISA Kit, acyl-CoA binding domain containing 6 ELISA Kit, acyl-CoA binding protein ELISA Kit, acyl-CoA binding domain containing 6 S homeolog ELISA Kit, acyl-CoA-binding protein 6 ELISA Kit, acyl-Coenzyme A binding domain containing 6 ELISA Kit, LHX4-AS1 ELISA Kit, ACBD6 ELISA Kit, acbd6 ELISA Kit, Acbd6 ELISA Kit, acbd6.S ELISA Kit, ACBP6 ELISA Kit

Gen-ID: 84320

UniProt: Q9BR61