14-3-3 theta ELISA Kit

Produktnummer ABIN6951519

Produktdetails 14-3-3 theta ELISA Kit

Target: 14-3-3 theta (YWHAQ)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human 14-3-3 theta ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Haltbarkeit: 6 months

Details zu 14-3-3 theta

Target: 14-3-3 theta (YWHAQ)

Andere Bezeichnung: 14-3-3 theta (YWHAQ Produkte)

Synonyme: 14-3-3 ELISA Kit, 14-3-3t ELISA Kit, 1c5 ELISA Kit, hs1 ELISA Kit, 1C5 ELISA Kit, HS1 ELISA Kit, 2700028P07Rik ELISA Kit, AA409740 ELISA Kit, AU021156 ELISA Kit, R74690 ELISA Kit, ywhaq ELISA Kit, ywhaq2 ELISA Kit, wu:fa91b01 ELISA Kit, wu:fb02e12 ELISA Kit, wu:fb36f06 ELISA Kit, wu:fk86h02 ELISA Kit, ywhaq1 ELISA Kit, ywhaz ELISA Kit, zgc:55499 ELISA Kit, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta ELISA Kit, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta ELISA Kit, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta S homeolog ELISA Kit, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide a ELISA Kit, 14-3-3 protein theta ELISA Kit, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide b ELISA Kit, YWHAQ ELISA Kit, ywhaq ELISA Kit, Ywhaq ELISA Kit, ywhaq.S ELISA Kit, ywhaqa ELISA Kit, ywhaqb ELISA Kit

Gen-ID: 10971

UniProt: P27348

Pathways: Apoptose, Myometrial Relaxation and Contraction