ATG4B ELISA Kit

Produktnummer ABIN6951529

Produktdetails ATG4B ELISA Kit

Target: ATG4B (Autophagy related 4B Cysteine Peptidase (ATG4B))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human ATG4B ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Haltbarkeit: 6 months

Details zu ATG4B

Target: ATG4B (Autophagy related 4B Cysteine Peptidase (ATG4B))

Andere Bezeichnung: ATG4B (ATG4B Produkte)

Synonyme: apg4b ELISA Kit, APG4B ELISA Kit, AUTL1 ELISA Kit, 2510009N07Rik ELISA Kit, AW048066 ELISA Kit, Apg4b ELISA Kit, Atg4bl ELISA Kit, Autl1 ELISA Kit, Aut2b2 ELISA Kit, autophagy related 4B cysteine peptidase ELISA Kit, autophagy-related 4b ELISA Kit, Peptidase family C54 protein ELISA Kit, cysteine protease ATG4B ELISA Kit, ATG4 autophagy related 4 homolog B (S. cerevisiae) ELISA Kit, autophagy related 4B, cysteine peptidase ELISA Kit, autophagy related 4B, cysteine peptidase L homeolog ELISA Kit, ATG4B ELISA Kit, Atg4b ELISA Kit, AT3G59950 ELISA Kit, Tsp_08582 ELISA Kit, Tsp_11599 ELISA Kit, atg4b ELISA Kit, atg4b.L ELISA Kit

Gen-ID: 23192

UniProt: Q9Y4P1

Pathways: Autophagie