ATG5 ELISA Kit

Produktnummer ABIN6951530

Produktdetails ATG5 ELISA Kit

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human ATG5 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Haltbarkeit: 6 months

Details zu ATG5

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Andere Bezeichnung: ATG5 (ATG5 Produkte)

Synonyme: APG5 ELISA Kit, APG5-LIKE ELISA Kit, APG5L ELISA Kit, ASP ELISA Kit, hAPG5 ELISA Kit, ATG5 ELISA Kit, CG1643 ELISA Kit, DmAtg5 ELISA Kit, Dmel\\CG1643 ELISA Kit, atg5 ELISA Kit, 2010107M05Rik ELISA Kit, 3110067M24Rik ELISA Kit, AW319544 ELISA Kit, Apg5l ELISA Kit, Atg5l ELISA Kit, C88337 ELISA Kit, Paddy ELISA Kit, apg5l ELISA Kit, zgc:100934 ELISA Kit, ATATG5 ELISA Kit, AUTOPHAGY 5 ELISA Kit, MKP11.20 ELISA Kit, MKP11_20 ELISA Kit, autophagy related 5 ELISA Kit, Autophagy-related 5 ELISA Kit, ATG5 autophagy related 5 homolog (S. cerevisiae) ELISA Kit, autophagy related 5 L homeolog ELISA Kit, similar to S. cerevisiae ATG5 (YPL149W) which nucleates preautophagosome formation as a conjugate with Atg12 ELISA Kit, autophagy protein Apg5 family ELISA Kit, ATG5 ELISA Kit, Atg5 ELISA Kit, atg5 ELISA Kit, atg5.L ELISA Kit, APG5 ELISA Kit

Gen-ID: 9474

UniProt: Q9H1Y0

Pathways: Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Autophagie