BOLA1 ELISA Kit

Produktnummer ABIN6951543

Produktdetails BOLA1 ELISA Kit

Target: BOLA1 (BolA-like Protein 1 (BOLA1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human BOLA1 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Haltbarkeit: 6 months

Details zu BOLA1

Target: BOLA1 (BolA-like Protein 1 (BOLA1))

Andere Bezeichnung: BOLA1 (BOLA1 Produkte)

Synonyme: 1810037G04Rik ELISA Kit, RGD1310586 ELISA Kit, BOLA1 ELISA Kit, zgc:101119 ELISA Kit, bolA family member 1 ELISA Kit, bolA-like 1 (E. coli) ELISA Kit, bolA homolog 1 (E. coli) ELISA Kit, transcriptional regulator BolA ELISA Kit, morphology/transcriptional regulatory protein BolA ELISA Kit, BolA-like protein ELISA Kit, bolA-like protein 1-like ELISA Kit, BOLA1 ELISA Kit, Bola1 ELISA Kit, bolA1 ELISA Kit, bola1 ELISA Kit, LOC100377069 ELISA Kit

Gen-ID: 51027

UniProt: Q9Y3E2