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SPEG ELISA Kit

SPEG Reaktivität: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Produktnummer ABIN6951561
  • Target Alle SPEG Produkte
    SPEG (SPEG Complex Locus (SPEG))
    Reaktivität
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Human SPEG ELISA Kit.
    Proben
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Haltbarkeit
    6 months
  • Target Alle SPEG Produkte
    SPEG (SPEG Complex Locus (SPEG))
    Andere Bezeichnung
    SPEG (SPEG Produkte)
    Synonyme
    apeg1 ELISA Kit, fc31c10 ELISA Kit, im:6912258 ELISA Kit, wu:fc31c10 ELISA Kit, APEG-1 ELISA Kit, APEG1 ELISA Kit, BPEG ELISA Kit, SPEGalpha ELISA Kit, SPEGbeta ELISA Kit, AW125581 ELISA Kit, Apeg1 ELISA Kit, D1Bwg1450e ELISA Kit, mKIAA1297 ELISA Kit, SPEG complex locus a ELISA Kit, SPEG complex locus ELISA Kit, spega ELISA Kit, SPEG ELISA Kit, Speg ELISA Kit
    Gen-ID
    10290
    UniProt
    Q15772
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