CES5A ELISA Kit

Produktnummer ABIN6951576

Produktdetails CES5A ELISA Kit

Target: CES5A (Carboxylesterase 5A (CES5A))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Mouse CES5A ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Haltbarkeit: 6 months

Details zu CES5A

Target: CES5A (Carboxylesterase 5A (CES5A))

Andere Bezeichnung: CES5A (CES5A Produkte)

Synonyme: CAUXIN ELISA Kit, CES4C1 ELISA Kit, CES5 ELISA Kit, CES7 ELISA Kit, 1700081L16Rik ELISA Kit, 1700122C07Rik ELISA Kit, BB081581 ELISA Kit, Ces7 ELISA Kit, Gm503 ELISA Kit, cauxin ELISA Kit, Ces5 ELISA Kit, LOC445455 ELISA Kit, carboxylesterase 5A ELISA Kit, CES5A ELISA Kit, Ces5a ELISA Kit

Gen-ID: 67935

UniProt: Q6AW46