CD57 ELISA Kit

Produktnummer ABIN6951578

Produktdetails CD57 ELISA Kit

Target: CD57 (B3GAT1) (beta-1,3-Glucuronyltransferase 1 (Glucuronosyltransferase P) (B3GAT1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human CD57/B3gat1 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Haltbarkeit: 6 months

Details zu CD57

Target: CD57 (B3GAT1) (beta-1,3-Glucuronyltransferase 1 (Glucuronosyltransferase P) (B3GAT1))

Andere Bezeichnung: CD57 (B3gat1 (B3GAT1 Produkte)

Synonyme: CD57 ELISA Kit, GLCATP ELISA Kit, GLCUATP ELISA Kit, HNK1 ELISA Kit, LEU7 ELISA Kit, NK-1 ELISA Kit, NK1 ELISA Kit, 0710007K08Rik ELISA Kit, AI846286 ELISA Kit, GlcAT-P ELISA Kit, Glcatp ELISA Kit, Hnk1 ELISA Kit, Hnk-1 ELISA Kit, b3gat1 ELISA Kit, si:dkey-22o20.1 ELISA Kit, cd57 ELISA Kit, glcatp ELISA Kit, glcuatp ELISA Kit, hnk-1 ELISA Kit, hnk1 ELISA Kit, leu7 ELISA Kit, nk-1 ELISA Kit, nk1 ELISA Kit, beta-1,3-glucuronyltransferase 1 ELISA Kit, beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P) ELISA Kit, beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P) a ELISA Kit, beta-1,3-glucuronyltransferase 1 L homeolog ELISA Kit, B3GAT1 ELISA Kit, B3gat1 ELISA Kit, b3gat1a ELISA Kit, b3gat1.L ELISA Kit

Gen-ID: 27087

UniProt: Q9P2W7

Pathways: Glycosaminoglycan Metabolic Process