SPARC ELISA Kit

Produktnummer ABIN6951624

Produktdetails SPARC ELISA Kit

Target: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human SPARC/Osteonectin ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Haltbarkeit: 6 months

Details zu SPARC

Target: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Andere Bezeichnung: SPARC (Osteonectin (SPARC Produkte)

Synonyme: ON ELISA Kit, AA517111 ELISA Kit, BM-40 ELISA Kit, hm:zeh0062 ELISA Kit, SPARC ELISA Kit, sparc ELISA Kit, secreted protein acidic and cysteine rich ELISA Kit, secreted acidic cysteine rich glycoprotein ELISA Kit, secreted protein, acidic, cysteine-rich (osteonectin) ELISA Kit, secreted protein, acidic, cysteine-rich (osteonectin) L homeolog ELISA Kit, secreted protein, acidic, cysteine-rich (osteonectin) S homeolog ELISA Kit, SPARC ELISA Kit, Sparc ELISA Kit, sparc ELISA Kit, sparc.L ELISA Kit, sparc.S ELISA Kit

Gen-ID: 6678

UniProt: P09486

Pathways: Autophagie