IP6K1 ELISA Kit

Produktnummer ABIN6951627

Produktdetails IP6K1 ELISA Kit

Target: IP6K1 (Inositol Hexakisphosphate Kinase 1 (IP6K1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human IP6K1 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Haltbarkeit: 6 months

Details zu IP6K1

Target: IP6K1 (Inositol Hexakisphosphate Kinase 1 (IP6K1))

Andere Bezeichnung: IP6K1 (IP6K1 Produkte)

Synonyme: ihpk1 ELISA Kit, MGC80337 ELISA Kit, IP6K1 ELISA Kit, IHPK1 ELISA Kit, pius ELISA Kit, DKFZp459D1030 ELISA Kit, Ihpk1 ELISA Kit, Itpk6 ELISA Kit, PiUS ELISA Kit, 1200016D08Rik ELISA Kit, AU022776 ELISA Kit, InsP6 ELISA Kit, InsP6k1 ELISA Kit, inositol hexaphosphate kinase 1 L homeolog ELISA Kit, inositol hexakisphosphate kinase 1 ELISA Kit, inositol hexaphosphate kinase 1 ELISA Kit, ip6k1.L ELISA Kit, IP6K1 ELISA Kit, ip6k1 ELISA Kit, Tsp_08677 ELISA Kit, Ip6k1 ELISA Kit

Gen-ID: 9807

UniProt: Q92551