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IL20RB ELISA Kit

IL20RB Reaktivität: Ratte Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Produktnummer ABIN6951643
  • Target Alle IL20RB ELISA Kits anzeigen
    IL20RB (Interleukin 20 Receptor beta (IL20RB))
    Reaktivität
    • 2
    • 1
    Ratte
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Rat IL-20 R beta ELISA Kit.
    Proben
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product IL20RB ELISA Kit
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Haltbarkeit
    6 months
  • Target Alle IL20RB ELISA Kits anzeigen
    IL20RB (Interleukin 20 Receptor beta (IL20RB))
    Andere Bezeichnung
    IL-20 R beta (IL20RB Produkte)
    Synonyme
    DIRS1 ELISA Kit, FNDC6 ELISA Kit, IL-20R2 ELISA Kit, AV228068 ELISA Kit, Fndc6 ELISA Kit, Gm186 ELISA Kit, Il20R2 ELISA Kit, IL20R2 ELISA Kit, RGD1566151 ELISA Kit, interleukin 20 receptor subunit beta ELISA Kit, interleukin 20 receptor beta ELISA Kit, IL20RB ELISA Kit, Il20rb ELISA Kit
    Gen-ID
    501043
    UniProt
    B8K1X9
    Pathways
    Regulation of Leukocyte Mediated Immunity
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