PSG1 ELISA Kit

Produktnummer ABIN6951686

Produktdetails PSG1 ELISA Kit

Target: PSG1 (Pregnancy Specific beta-1-Glycoprotein 1 (PSG1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human PSG1 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Haltbarkeit: 6 months

Details zu PSG1

Target: PSG1 (Pregnancy Specific beta-1-Glycoprotein 1 (PSG1))

Andere Bezeichnung: PSG1 (PSG1 Produkte)

Synonyme: B1G1 ELISA Kit, CD66f ELISA Kit, DHFRP2 ELISA Kit, FL-NCA-1/2 ELISA Kit, PBG1 ELISA Kit, PS-beta-C/D ELISA Kit, PS-beta-G-1 ELISA Kit, PSBG-1 ELISA Kit, PSBG1 ELISA Kit, PSG95 ELISA Kit, PSGGA ELISA Kit, PSGIIA ELISA Kit, SP1 ELISA Kit, PSG1 ELISA Kit, PSG10 ELISA Kit, PSG12 ELISA Kit, Psg ELISA Kit, pregnancy specific beta-1-glycoprotein 1 ELISA Kit, pregnancy specific beta-1-glycoprotein 2 ELISA Kit, pregnancy specific beta-1-glycoprotein 10, pseudogene ELISA Kit, pregnancy-specific beta 1-glycoprotein ELISA Kit, PSG1 ELISA Kit, PSG2 ELISA Kit, PSG10P ELISA Kit, Psgb1 ELISA Kit

Gen-ID: 5669

UniProt: P11464