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SETD7 ELISA Kit

SETD7 Reaktivität: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Produktnummer ABIN6951695
  • Target Alle SETD7 ELISA Kits anzeigen
    SETD7 (SET Domain Containing (Lysine Methyltransferase) 7 (SETD7))
    Reaktivität
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Human SETD7 ELISA Kit.
    Proben
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product SETD7 ELISA Kit
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Haltbarkeit
    6 months
  • Target Alle SETD7 ELISA Kits anzeigen
    SETD7 (SET Domain Containing (Lysine Methyltransferase) 7 (SETD7))
    Andere Bezeichnung
    SETD7 (SETD7 Produkte)
    Synonyme
    KMT7 ELISA Kit, SET7 ELISA Kit, SET7/9 ELISA Kit, SET9 ELISA Kit, set7 ELISA Kit, zgc:101860 ELISA Kit, zgc:92330 ELISA Kit, 1600028F23Rik ELISA Kit, H3K4MT ELISA Kit, Set7 ELISA Kit, Set7/9 ELISA Kit, mKIAA1717 ELISA Kit, SET domain containing lysine methyltransferase 7 ELISA Kit, SET domain containing (lysine methyltransferase) 7 ELISA Kit, SETD7 ELISA Kit, setd7 ELISA Kit, Setd7 ELISA Kit
    Gen-ID
    80854
    UniProt
    Q8WTS6
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