TSC22D1 ELISA Kit
TSC22D1
Reaktivität: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Produktnummer ABIN6951701
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Target
Alle TSC22D1 ELISA Kits anzeigen
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TSC22D1
(TSC22 Domain Family, Member 1 (TSC22D1))
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Reaktivität
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Human
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Nachweismethode
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Colorimetric
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Methodentyp
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Sandwich ELISA
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Applikation
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ELISA
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Verwendungszweck
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Human TSC22D1 ELISA Kit.
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Proben
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Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Analytische Methode
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Quantitative
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Produktmerkmale
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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Bestandteile
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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Benötigtes Material
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Top Product
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Discover our top product TSC22D1 ELISA Kit
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Applikationshinweise
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Optimal working dilution should be determined by the investigator.
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Plattentyp
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Pre-coated
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Protokoll
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Haltbarkeit
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6 months
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Target
Alle TSC22D1 ELISA Kits anzeigen
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TSC22D1
(TSC22 Domain Family, Member 1 (TSC22D1))
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Andere Bezeichnung
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TSC22D1 (TSC22D1 Produkte)
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Synonyme
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Ptg-2 ELISA Kit, TGFB1I4 ELISA Kit, TSC22 ELISA Kit, AA589566 ELISA Kit, AW105905 ELISA Kit, Egr5 ELISA Kit, TSC-22 ELISA Kit, Tgfb1i4 ELISA Kit, Tsc ELISA Kit, Tsc22 ELISA Kit, tsc22 ELISA Kit, TS22A ELISA Kit, fj34d05 ELISA Kit, wu:fj34d05 ELISA Kit, zgc:65869 ELISA Kit, zgc:85857 ELISA Kit, TSC22D1 ELISA Kit, tsc22d1 ELISA Kit, MGC69210 ELISA Kit, MGC186361 ELISA Kit, DKFZp459J163 ELISA Kit, TSC22 domain family member 1 ELISA Kit, TSC22 domain family, member 1 ELISA Kit, TSC22 domain family member 1 L homeolog ELISA Kit, TSC22D1 ELISA Kit, Tsc22d1 ELISA Kit, tsc22d1.L ELISA Kit, tsc22d1 ELISA Kit
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Gen-ID
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8848
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UniProt
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Q15714
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