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TSC22D1 ELISA Kit

TSC22D1 Reaktivität: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Produktnummer ABIN6951701
  • Target Alle TSC22D1 ELISA Kits anzeigen
    TSC22D1 (TSC22 Domain Family, Member 1 (TSC22D1))
    Reaktivität
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Human TSC22D1 ELISA Kit.
    Proben
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product TSC22D1 ELISA Kit
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Haltbarkeit
    6 months
  • Target Alle TSC22D1 ELISA Kits anzeigen
    TSC22D1 (TSC22 Domain Family, Member 1 (TSC22D1))
    Andere Bezeichnung
    TSC22D1 (TSC22D1 Produkte)
    Synonyme
    Ptg-2 ELISA Kit, TGFB1I4 ELISA Kit, TSC22 ELISA Kit, AA589566 ELISA Kit, AW105905 ELISA Kit, Egr5 ELISA Kit, TSC-22 ELISA Kit, Tgfb1i4 ELISA Kit, Tsc ELISA Kit, Tsc22 ELISA Kit, tsc22 ELISA Kit, TS22A ELISA Kit, fj34d05 ELISA Kit, wu:fj34d05 ELISA Kit, zgc:65869 ELISA Kit, zgc:85857 ELISA Kit, TSC22D1 ELISA Kit, tsc22d1 ELISA Kit, MGC69210 ELISA Kit, MGC186361 ELISA Kit, DKFZp459J163 ELISA Kit, TSC22 domain family member 1 ELISA Kit, TSC22 domain family, member 1 ELISA Kit, TSC22 domain family member 1 L homeolog ELISA Kit, TSC22D1 ELISA Kit, Tsc22d1 ELISA Kit, tsc22d1.L ELISA Kit, tsc22d1 ELISA Kit
    Gen-ID
    8848
    UniProt
    Q15714
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