TLE3 ELISA Kit

Produktnummer ABIN6951703

Produktdetails TLE3 ELISA Kit

Target: TLE3 (Transducin-Like Enhancer Protein 3 (TLE3))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human TLE3 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Haltbarkeit: 6 months

Details zu TLE3

Target: TLE3 (Transducin-Like Enhancer Protein 3 (TLE3))

Andere Bezeichnung: TLE3 (TLE3 Produkte)

Synonyme: TLE3 ELISA Kit, ESG ELISA Kit, ESG1 ELISA Kit, Xesg1 ELISA Kit, esg2 ELISA Kit, esg3 ELISA Kit, grg2 ELISA Kit, grg3 ELISA Kit, hst18976 ELISA Kit, tle3 ELISA Kit, xgrg3 ELISA Kit, ESG3 ELISA Kit, GRG3 ELISA Kit, HsT18976 ELISA Kit, Esp3 ELISA Kit, 2610103N05Rik ELISA Kit, Grg3a ELISA Kit, Grg3b ELISA Kit, mKIAA1547 ELISA Kit, transducin like enhancer of split 3 ELISA Kit, transducin like enhancer of split 2 ELISA Kit, transducin-like enhancer of split 3 ELISA Kit, TLE3 ELISA Kit, tle2 ELISA Kit, Tle3 ELISA Kit

Gen-ID: 7090

UniProt: Q04726

Pathways: SARS-CoV-2 Protein Interaktom