GFPT1 ELISA Kit

Produktnummer ABIN6951707

Produktdetails GFPT1 ELISA Kit

Target: GFPT1 (Glutamine--Fructose-6-Phosphate Transaminase 1 (GFPT1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human GFPT1 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Haltbarkeit: 6 months

Details zu GFPT1

Target: GFPT1 (Glutamine--Fructose-6-Phosphate Transaminase 1 (GFPT1))

Andere Bezeichnung: GFPT1 (GFPT1 Produkte)

Synonyme: gfat ELISA Kit, 2810423A18Rik ELISA Kit, AI324119 ELISA Kit, AI449986 ELISA Kit, GFA ELISA Kit, GFAT ELISA Kit, GFAT1 ELISA Kit, GFAT1m ELISA Kit, Gfpt ELISA Kit, CMSTA1 ELISA Kit, GFAT 1 ELISA Kit, GFPT ELISA Kit, GFPT1L ELISA Kit, MSLG ELISA Kit, glutamine--fructose-6-phosphate transaminase 1 ELISA Kit, glutamine--fructose-6-phosphate transaminase 1 S homeolog ELISA Kit, glutamine fructose-6-phosphate transaminase 1 ELISA Kit, GFPT1 ELISA Kit, gfpt1.S ELISA Kit, Gfpt1 ELISA Kit

Gen-ID: 2673

UniProt: Q06210

Pathways: ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process