WARS ELISA Kit

Produktnummer ABIN6951745

Produktdetails WARS ELISA Kit

Target: WARS (Tryptophanyl-tRNA Synthetase (WARS))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human WARS ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Haltbarkeit: 6 months

Details zu WARS

Target: WARS (Tryptophanyl-tRNA Synthetase (WARS))

Andere Bezeichnung: WARS (WARS Produkte)

Synonyme: GAMMA-2 ELISA Kit, IFI53 ELISA Kit, IFP53 ELISA Kit, zgc:73274 ELISA Kit, wars ELISA Kit, MGC53671 ELISA Kit, WARS ELISA Kit, TrpRS ELISA Kit, WRS ELISA Kit, 85D-WRS ELISA Kit, CG9735 ELISA Kit, Dmel\\CG9735 ELISA Kit, WRS-85D ELISA Kit, l(3)03559 ELISA Kit, l(3)03560 ELISA Kit, l(3)3559 ELISA Kit, gamma-2 ELISA Kit, ifi53 ELISA Kit, ifp53 ELISA Kit, GB14934 ELISA Kit, ECK3371 ELISA Kit, JW3347 ELISA Kit, tryptophanyl-tRNA synthetase ELISA Kit, tryptophanyl-tRNA synthetase S homeolog ELISA Kit, Tryptophanyl-tRNA synthetase ELISA Kit, tryptophanyl-tRNA synthetase L homeolog ELISA Kit, tryptophan--tRNA ligase, cytoplasmic ELISA Kit, WARS ELISA Kit, wars ELISA Kit, wars.S ELISA Kit, Wars ELISA Kit, TrpRS ELISA Kit, wars.L ELISA Kit, LOC727582 ELISA Kit, trpS ELISA Kit, trpS2 ELISA Kit

Gen-ID: 7453

UniProt: P23381