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CBX5 ELISA Kit

CBX5 Reaktivität: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Produktnummer ABIN6951753
  • Target Alle CBX5 ELISA Kits anzeigen
    CBX5 (Chromobox Homolog 5 (CBX5))
    Reaktivität
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Human CBX5 ELISA Kit.
    Proben
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product CBX5 ELISA Kit
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Haltbarkeit
    6 months
  • Target Alle CBX5 ELISA Kits anzeigen
    CBX5 (Chromobox Homolog 5 (CBX5))
    Andere Bezeichnung
    CBX5 (CBX5 Produkte)
    Synonyme
    HP1 ELISA Kit, HP1A ELISA Kit, id:ibd2026 ELISA Kit, wu:fc63e02 ELISA Kit, wu:fe47g12 ELISA Kit, wu:fi03h05 ELISA Kit, zgc:158601 ELISA Kit, CBX5 ELISA Kit, cbx5 ELISA Kit, MGC64354 ELISA Kit, 2610029O15Rik ELISA Kit, C75991 ELISA Kit, Hp1a ELISA Kit, Hp1alpha ELISA Kit, chromobox 5 ELISA Kit, chromobox homolog 5 (HP1 alpha homolog, Drosophila) ELISA Kit, chromobox 5 S homeolog ELISA Kit, CBX5 ELISA Kit, cbx5 ELISA Kit, Cbx5 ELISA Kit, cbx5.S ELISA Kit
    Gen-ID
    23468
    UniProt
    P45973
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