EDAR ELISA Kit

Produktnummer ABIN6951754

Produktdetails EDAR ELISA Kit

Target: EDAR (Ectodysplasin A Receptor (EDAR))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human EDAR ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Haltbarkeit: 6 months

Details zu EDAR

Target: EDAR (Ectodysplasin A Receptor (EDAR))

Andere Bezeichnung: EDAR (EDAR Produkte)

Synonyme: rs3 ELISA Kit, edar ELISA Kit, EDAR ELISA Kit, MGC88893 ELISA Kit, DL ELISA Kit, ECTD10A ELISA Kit, ECTD10B ELISA Kit, ED1R ELISA Kit, ED3 ELISA Kit, ED5 ELISA Kit, EDA-A1R ELISA Kit, EDA1R ELISA Kit, EDA3 ELISA Kit, HRM1 ELISA Kit, dl ELISA Kit, RGD1561714 ELISA Kit, ectodysplasin A receptor ELISA Kit, ectodysplasin-A receptor ELISA Kit, edar ELISA Kit, EDAR ELISA Kit, Edar ELISA Kit

Gen-ID: 10913

UniProt: Q9UNE0

Pathways: Tube Formation, Ubiquitin Proteasome Pathway