FSH ELISA Kit

Produktnummer ABIN6955956

Produktdetails FSH ELISA Kit

Target: FSH (Follicle-Stimulating Hormone (FSH))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Competition ELISA

Detektionsbereich: 2.22 ng/mL - 180 ng/mL

Untere Nachweisgrenze: 2.22 ng/mL

Applikation: ELISA

Verwendungszweck: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of FSH in mouse serum, plasma.

Proben: Plasma, Serum

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Follicle Stimulating Hormone (FSH)

Sensitivität: 0.84 ng/mL

Bestandteile:

• Pre-coated, ready to use 96-well strip plate, flat buttom
• Plate sealer for 96 wells
• Reference Standard
• Standard Diluent
• Detection Reagent A
• Detection Reagent B
• Assay Diluent A
• Assay Diluent B
• Reagent Diluent (if Detection Reagent is lyophilized)
• TMB Substrate
• Stop Solution
• Wash Buffer (30 x concentrate)
• Instruction manual

Anwendungsinformationen

Kommentare:

Information on standard material:
The standard might be recombinant protein or natural protein, that will depend on the specific kit. Moreover, the expression system is E.coli or yeast or mammal cell. There is 0.05% proclin 300 in the standard as preservative.

Information on reagents:
The stop solution used in the kit is sulfuric acid with concentration of 1 mol/L. And the wash solution is TBS. The standard diluent contains 0.02 % sodium azide, assay diluent A and assay diluent B contain 0.01% sodium azide. Some kits can contain is BSA in them.

Information on antibodies:
The provided antibodies and their host vary in different kits.

Probenmenge: 50 μL

Testdauer: 2 h

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards,
2. Add 50μL standard or sample to each well.
   Then add 50μL prepared Detection Reagent A immediately.
   Shake and mix. Incubate 1 hour at 37 °C,
3. Aspirate and wash 3 times,
4. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C,
5. Aspirate and wash 5 times,
6. Add 90μL Substrate Solution. Incubate 10-20 minutes at 37 °C,
7. Add 50μL Stop Solution. Read at 450 nm immediately.

Aufbereitung der Reagenzien:

1. Bring all kit components and samples to room temperature (18-25 °C) before use. If the kit will not be used up in one time, please only take out strips and reagents for present experiment, and leave the remaining strips and reagents in required condition.
2. Standard - Reconstitute the Standard with 1.0 mL of Standard Diluent, kept for 10 minutes at room temperature, shake gently(not to foam). The concentration of the standard in the stock solution is 540 ng/mL. Please firstly dilute the stock solution to 180 ng/mL and the diluted standard serves as the highest standard (180 ng/mL). Then prepare 5 tubes containing 0.6 mL Standard Diluent and produce a triple dilution series according to the picture shown below. Mix each tube thoroughly before the next transfer. Set up 5 points of diluted standard such as 180 ng/mL, 60 ng/mL, 20 ng/mL, 6.67 ng/mL, 2.22 ng/mL, and the last EP tubes with Standard Diluent is the blank as 0 ng/mL.
3. Detection Reagent A and Detection Reagent B - If lyophilized reconstitute the Detection Reagent A with 150μL of Reagent Diluent, kept for 10 minutes at room temperature, shake gently (not to foam). Briefly spin or centrifuge the stock Detection A and Detection B before use. Dilute them to the working concentration 100-fold with Assay Diluent A and B, respectively.
4. Wash Solution - Dilute 20 mL of Wash Solution concentrate (30x) with 580 mL of deionized or distilled water to prepare 600 mL of Wash Solution (1x).
5. TMB substrate - Aspirate the needed dosage of the solution with sterilized tips and do not dump the residual solution into the vial again.

Note:

1. Making serial dilution in the wells directly is not permitted.
2. Prepare standard within 15 minutes before assay. Please do not dissolve the reagents at 37 °C directly.
3. Detection Reagent A and B are sticky solutions, therefore, slowly pipette them to reduce the volume errors.
4. Please carefully reconstitute Standards or working Detection Reagent A and B according to the instruction, and avoid foaming and mix gently until the crystals are completely dissolved. To minimize imprecision caused by pipetting, use small volumes and ensure that pipettors are calibrated. It is recommended to suck more than 10μL for one pipetting.
5. The reconstituted Standards, Detection Reagent A and Detection Reagent B can be used only once.
6. If crystals have formed in the Wash Solution concentrate (30x), warm to room temperature and mix gently until the crystals are completely dissolved.
7. Contaminated water or container for reagent preparation will influence the detection result.

Aufbereitung der Proben:

• It is recommended to use fresh samples without long storage, otherwise protein degradation and denaturation may occur in these samples, leading to false results. Samples should therefore be stored for a short period at 2 - 8 °C or aliquoted at -20 °C (≤1 month) or -80 °C (≤ 3 months). Repeated freeze-thaw cycles should be avoided. Prior to assay, the frozen samples should be slowly thawed and centrifuged to remove precipitates.
• If the sample type is not specified in the instructions, a preliminary test is necessary to determine compatibility with the kit.
• If a lysis buffer is used to prepare tissue homogenates or cell culture supernatant, there is a possibility of causing a deviation due to the introduced chemical substance. The recommended dilution factor is for reference only.
• Please estimate the concentration of the samples before performing the test. If the values are not in the range of the standard curve, the optimal sample dilution for the particular experiment has to be determined.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level of target were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level of target were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV < 10%
Inter-Assay: CV < 12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Vorsichtsmaßnahmen: The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung:

1. For unopened kit: All reagents should be stored according to the labels on the vials. The Standard, Detection Reagent A, Detection Reagent B, and 96-well Strip Plate should be stored at -20 °C upon receipt, while the other reagents should be stored at 4 °C.
2. For opened kits: the remaining reagents must be stored according to the above storage conditions. In addition, please return the unused wells to the foil pouch containing the desiccant and seal the foil pouch with the zipper.

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Haltbarkeit: 6 months

Referenzen für FSH ELISA Kit (ABIN6955956)

Li, Ma, Liu: "Pyrethroid Insecticide Cypermethrin Modulates Gonadotropin Synthesis via Calcium Homeostasis and ERK1/2 Signaling in LβT2 Mouse Pituitary Cells." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 162, Issue 1, pp. 43-52, (2019) (PubMed).

Jones, Lang, Kendziorski, Greene, Burns: "Use of a Mouse Model of Experimentally Induced Endometriosis to Evaluate and Compare the Effects of Bisphenol A and Bisphenol AF Exposure." in: Environmental health perspectives, Vol. 126, Issue 12, pp. 127004, (2019) (PubMed).

Fu, Chen, Xu, Ji, Zhang, Wang, Yu, Xu: "Vitamin D deficiency impairs testicular development and spermatogenesis in mice." in: Reproductive toxicology (Elmsford, N.Y.), Vol. 73, pp. 241-249, (2018) (PubMed).

Ding, Tan, Song, Ma, Xiao, Zhang: "Effect of controlled ovarian hyperstimulation on puberty and estrus in mice offspring." in: Reproduction (Cambridge, England), Vol. 154, Issue 4, pp. 433-444, (2018) (PubMed).

Ye, Li, Zhang, Ma, Ji, Huang, Curry, Liu, Liu: "Pyrethroid Insecticide Cypermethrin Accelerates Pubertal Onset in Male Mice via Disrupting Hypothalamic-Pituitary-Gonadal Axis." in: Environmental science & technology, Vol. 51, Issue 17, pp. 10212-10221, (2018) (PubMed).

Wang, Wang, Lu, Cui, Li, Li, Zhang, Zhang, Liu: "The Combination of icariin and constrained dynamic loading stimulation attenuates bone loss in ovariectomy-induced osteoporotic mice." in: Journal of orthopaedic research : official publication of the Orthopaedic Research Society, Vol. 36, Issue 5, pp. 1415-1424, (2018) (PubMed).

Cao, Hua, Xiong, Zhu, Zhang, Chen: "Impact of Triclosan on Female Reproduction through Reducing Thyroid Hormones to Suppress Hypothalamic Kisspeptin Neurons in Mice." in: Frontiers in molecular neuroscience, Vol. 11, pp. 6, (2018) (PubMed).

Tang, Yin, Zhang, Chen, Jin, Liu: "Prenatal exposure to polychlorinated biphenyl and umbilical cord hormones and birth outcomes in an island population." in: Environmental pollution (Barking, Essex : 1987), Vol. 237, pp. 581-591, (2018) (PubMed).

Varamini, Mansfeld, Giddam, Steyn, Toth: "New gonadotropin-releasing hormone glycolipids with direct antiproliferative activity and gonadotropin-releasing potency." in: International journal of pharmaceutics, Vol. 521, Issue 1-2, pp. 327-336, (2017) (PubMed).

Miura, Ohtani, Hasegawa, Yoshioka, Hwang: "High sensitivity of testicular function to titanium nanoparticles." in: The Journal of toxicological sciences, Vol. 42, Issue 3, pp. 359-366, (2017) (PubMed).

Iizuka-Hishikawa, Hishikawa, Sasaki, Takubo, Goto, Nagata, Nakanishi, Shindou, Okamura, Ito, Toshimori, Sasaki, Shimizu: "Lysophosphatidic acid acyltransferase 3 tunes the membrane status of germ cells by incorporating docosahexaenoic acid during spermatogenesis." in: The Journal of biological chemistry, Vol. 292, Issue 29, pp. 12065-12076, (2017) (PubMed).

Varamini, Rafiee, Giddam, Mansfeld, Steyn, Toth: "Development of New Gonadotropin-Releasing Hormone-Modified Dendrimer Platforms with Direct Antiproliferative and Gonadotropin Releasing Activity." in: Journal of medicinal chemistry, Vol. 60, Issue 20, pp. 8309-8320, (2017) (PubMed).

Choi, Lee, Ku, Cho, Seo, Lee, Lee: "FoxO1 is a negative regulator of FSH? gene expression in basal and GnRH-stimulated conditions in female." in: Endocrinology, Vol. 155, Issue 6, pp. 2277-86, (2014) (PubMed).

Liu, Tao, Lu, Yang, Ma, Zhang: "Targeted disruption of the mouse testis-enriched gene Znf230 does not affect spermatogenesis or fertility." in: Genetics and molecular biology, Vol. 37, Issue 4, pp. 708-15, (2014) (PubMed).

Details zu FSH

Target: FSH (Follicle-Stimulating Hormone (FSH))

Andere Bezeichnung: Follicle Stimulating Hormone (FSH) (FSH Produkte)

Synonyme: FSH ELISA Kit, Fshbeta ELISA Kit, follicle stimulating hormone beta ELISA Kit, Plasma FSH concentration ELISA Kit, Fshb ELISA Kit, FSH ELISA Kit

Substanzklasse: Hormone

Pathways: Peptide Hormone Metabolism, Chromatin Binding