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NT-ProBNP ELISA Kit

NT-ProBNP Reaktivität: Human Colorimetric Sandwich ELISA 39 pg/mL - 2500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Produktnummer ABIN6958739
  • Target Alle NT-ProBNP ELISA Kits anzeigen
    NT-ProBNP (Pro-Brain Natriuretic Peptide (NT-ProBNP) (NT-ProBNP))
    Reaktivität
    • 8
    • 7
    • 7
    • 6
    • 5
    • 5
    • 3
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    39 pg/mL - 2500 pg/mL
    Untere Nachweisgrenze
    39 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of NT-ProBNP in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates.
    Proben
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP)
    Sensitivität
    15 pg/mL
    Bestandteile
    • Pre-coated, ready to use 96-well strip plate, flat buttom
    • Plate sealer for 96 wells
    • Reference Standard
    • Standard Diluent
    • Detection Reagent A
    • Detection Reagent B
    • Assay Diluent A
    • Assay Diluent B
    • Reagent Diluent (if Detection Reagent is lyophilized)
    • TMB Substrate
    • Stop Solution
    • Wash Buffer (30 x concentrate)
    • Instruction manual
    Top Product
    Discover our top product NT-ProBNP ELISA Kit
  • Kommentare

    Information on standard material:
    The standard might be recombinant protein or natural protein, that will depend on the specific kit. Moreover, the expression system is E.coli or yeast or mammal cell. There is 0.05% proclin 300 in the standard as preservative.

    Information on reagents:
    The stop solution used in the kit is sulfuric acid with concentration of 1 mol/L. And the wash solution is TBS. The standard diluent contains 0.02 % sodium azide, assay diluent A and assay diluent B contain 0.01% sodium azide. Some kits can contain is BSA in them.

    Information on antibodies:
    The provided antibodies and their host vary in different kits.

    Probenmenge
    100 μL
    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards,
    2. Add 100μL standard or sample to each well. Incubate 1 hours at 37 °C,
    3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37 °C,
    4. Aspirate and wash 3 times,
    5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C,
    6. Aspirate and wash 5 times,
    7. Add 90μL Substrate Solution. Incubate 10-20 minutes at 37 °C,
    8. Add 50μL Stop Solution. Read at 450nm immediately.
    Aufbereitung der Reagenzien
    1. Bring all kit components and samples to room temperature (18-25 °C) before use. If the kit will not be used up in one time, please only take out strips and reagents for present experiment, and leave the remaining strips and reagents in required condition.
    2. Standard - Reconstitute the Standard with 0.5mL of Standard Diluent, kept for 10 minutes at room temperature, shake gently (not to foam). The concentration of the standard in the stock solution is 2,500pg/mL. Prepare 7 tubes containing 0.25mL Standard Diluent and produce a double dilution series. Mix each tube thoroughly before the next transfer. Set up 7 points of diluted standard such as 2,500pg/mL, 1,250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL, 78pg/mL, 39pg/mL, and the last tube with Standard Diluent is the blank as 0pg/ml.
    3. Detection Reagent A and Detection Reagent B - If lyophilized reconstitute the Detection Reagent A with 150μL of Reagent Diluent, keep for 10 minutes at room temperature, shake gently (not to foam). Briefly spin or centrifuge the stock Detection A and Detection B before use. Dilute them to the working concentration 100-fold with Assay Diluent A and B, respectively.
    4. Wash Solution - Dilute 20 mL of Wash Solution concentrate (30x) with 580 mL of deionized or distilled water to prepare 600 mL of Wash Solution (1x).
    5. TMB substrate - Aspirate the needed dosage of the solution with sterilized tips and do not dump the residual solution into the vial again.

    Note:

    1. Making serial dilution in the wells directly is not permitted.
    2. Prepare standards within 15 minutes before assay. Please do not dissolve the reagents at 37 °C directly.
    3. Please carefully reconstitute Standards or working Detection Reagent A and B according to the instruction, and avoid foaming and mix gently until the crystals are completely dissolved. To minimize imprecision caused by pipetting, use small volumes and ensure that pipettors are calibrated. It is recommended to suck more than 10μL for one pipetting.
    4. The reconstituted Standards, Detection Reagent A and Detection Reagent B can be used only once.
    5. If crystals have formed in the Wash Solution concentrate (30x), warm to room temperature and mix gently until the crystals are completely dissolved.
    6. Contaminated water or container for reagent preparation will influence the detection result.
    Aufbereitung der Proben
    • It is recommended to use fresh samples without long storage, otherwise protein degradation and denaturation may occur in these samples, leading to false results. Samples should therefore be stored for a short period at 2 - 8 °C or aliquoted at -20 °C (≤1 month) or -80 °C (≤ 3 months). Repeated freeze-thaw cycles should be avoided. Prior to assay, the frozen samples should be slowly thawed and centrifuged to remove precipitates.
    • If the sample type is not specified in the instructions, a preliminary test is necessary to determine compatibility with the kit.
    • If a lysis buffer is used to prepare tissue homogenates or cell culture supernatant, there is a possibility of causing a deviation due to the introduced chemical substance. The recommended dilution factor is for reference only.
    • Please estimate the concentration of the samples before performing the test. If the values are not in the range of the standard curve, the optimal sample dilution for the particular experiment has to be determined.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level of target were tested 20 times on one plate, respectively.
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level of target were tested on 3 different plates, 8 replicates in each plate.
    CV(%) = SD/meanX100
    Intra-Assay: CV < 10%
    Inter-Assay: CV < 12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Vorsichtsmaßnahmen
    The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
    Lagerung
    4 °C/-20 °C
    Informationen zur Lagerung
    1. For unopened kit: All reagents should be stored according to the labels on the vials. The Standard, Detection Reagent A, Detection Reagent B, and 96-well Strip Plate should be stored at -20 °C upon receipt, while the other reagents should be stored at 4 °C.
    2. For opened kits: the remaining reagents must be stored according to the above storage conditions. In addition, please return the unused wells to the foil pouch containing the desiccant and seal the foil pouch with the zipper.
    .
    Haltbarkeit
    6 months
  • Büttner, Schumacher, Dinov, Zeynalova, Sommer, Bollmann, Husser, Hindricks, Kornej: "Role of NT-proANP and NT-proBNP in patients with atrial fibrillation: Association with atrial fibrillation progression phenotypes." in: Heart rhythm, Vol. 15, Issue 8, pp. 1132-1137, (2019) (PubMed).

    Heidrich, Melz, Buechau, Pfluecke, Quick, Speiser, Poitz, Augstein, Ruf, Wässnig, Youssef, Strasser, Wiedemann: "Regulation of circulating chromogranin B levels in heart failure." in: Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals, Vol. 23, Issue 1, pp. 78-87, (2018) (PubMed).

    Marketou, Kontaraki, Maragkoudakis, Patrianakos, Konstantinou, Nakou, Vougia, Logakis, Chlouverakis, Vardas, Parthenakis: "MicroRNAs in Peripheral Mononuclear Cells as Potential Biomarkers in Hypertensive Patients With Heart Failure With Preserved Ejection Fraction." in: American journal of hypertension, Vol. 31, Issue 6, pp. 651-657, (2018) (PubMed).

    Pathak, Aris, Jensen, Huang, Ford: "Effect of 6-min Walk Test on pro-BNP Levels in Patients with Pulmonary Arterial Hypertension." in: Lung, Vol. 196, Issue 3, pp. 315-319, (2018) (PubMed).

    Baillie, Sidharta, Steele, Worthley, Willoughby, Teo, Sanders, Nicholls, Worthley et al.: "The predictive capabilities of a novel cardiovascular magnetic resonance derived marker of cardiopulmonary reserve on established prognostic surrogate markers in patients with pulmonary vascular ..." in: Journal of cardiovascular magnetic resonance : official journal of the Society for Cardiovascular Magnetic Resonance, Vol. 19, Issue 1, pp. 3, (2017) (PubMed).

    Buyuklu, Kandemir, Set, Bakırcı, Degirmenci, Hamur, Topal, Kucukler, Turkmen: "Beneficial Effects of Ozone Therapy on Oxidative Stress, Cardiac Functions and Clinical Findings in Patients with Heart Failure Reduced Ejection Fraction." in: Cardiovascular toxicology, (2017) (PubMed).

    Chiu, Ma, Lin, Lo, Lin, Wu, Chiang, Lee, Hung, Wang, Daisy Liu, Ho, Lin, Peng: "Serial heart rhythm complexity changes in patients with anterior wall ST segment elevation myocardial infarction." in: Scientific reports, Vol. 7, pp. 43507, (2017) (PubMed).

    Priya, Siddiqi, Karoli, Fatima, Gupta, Mishra: "Study of Vitamin D Status in Patients with Dilated Cardiomyopathy at a Teaching Hospital in North India." in: Journal of cardiovascular echography, Vol. 26, Issue 3, pp. 89-93, (2017) (PubMed).

    Iskandar, Liu, Xiang, Chen, Li, Qin, Huang, Chen: "Expression of pericardial fluid T-cells and related inflammatory cytokines in patients with chronic heart failure." in: Experimental and therapeutic medicine, Vol. 13, Issue 5, pp. 1850-1858, (2017) (PubMed).

    Liu, Iskandar, Chen, Zhang, Wang, Chen, Xiang: "Soluble Glycoprotein 130 and Heat Shock Protein 27 as Novel Candidate Biomarkers of Chronic Heart Failure with Preserved Ejection Fraction." in: Heart, lung & circulation, Vol. 25, Issue 10, pp. 1000-6, (2016) (PubMed).

    Lin, Kuo, Lee, Chou, Chen, Yeh, Huang, Hung, Liu, Ho, Wu: "5-methoxytryptophan is a potential marker for post-myocardial infarction heart failure - a preliminary approach to clinical utility." in: International journal of cardiology, Vol. 222, pp. 895-900, (2016) (PubMed).

    Bielecka-Dabrowa, Sakowicz, Misztal, von Haehling, Ahmed, Pietrucha, Rysz, Banach: "Differences in biochemical and genetic biomarkers in patients with heart failure of various etiologies." in: International journal of cardiology, Vol. 221, pp. 1073-80, (2016) (PubMed).

    Wu, Yang, Hung, Tsai, Hu, Lin, Chu, Wu, Wu: "Effect of Treatment on Body Fluid in Patients with Unilateral Aldosterone Producing Adenoma: Adrenalectomy versus Spironolactone." in: Scientific reports, Vol. 5, pp. 15297, (2015) (PubMed).

    Bielecka-Dabrowa, Michalska-Kasiczak, Gluba, Ahmed, Gerdts, von Haehling, Rysz, Banach: "Biomarkers and echocardiographic predictors of myocardial dysfunction in patients with hypertension." in: Scientific reports, Vol. 5, pp. 8916, (2015) (PubMed).

    Bielecka-Dabrowa, Gluba-Brzózka, Michalska-Kasiczak, Misztal, Rysz, Banach: "The multi-biomarker approach for heart failure in patients with hypertension." in: International journal of molecular sciences, Vol. 16, Issue 5, pp. 10715-33, (2015) (PubMed).

    Wiernicki, Rozwadowska, Malcher, Kolanowski, Zimna, Rugowska, Kurpisz: "Human myoblast transplantation in mice infarcted heart alters the expression profile of cardiac genes associated with left ventricle remodeling." in: International journal of cardiology, Vol. 202, pp. 710-21, (2015) (PubMed).

    Clavel, Côté, Mathieu, Dumesnil, Audet, Pépin, Couture, Fournier, Trahan, Pagé, Pibarot: "Paradoxical low-flow, low-gradient aortic stenosis despite preserved left ventricular ejection fraction: new insights from weights of operatively excised aortic valves." in: European heart journal, Vol. 35, Issue 38, pp. 2655-62, (2014) (PubMed).

    Krishna, Pal, Pal, Balachander, Jayasettiaseelon, Sreekanth, Sridhar, Gaur: "A Randomized Controlled Trial to Study the Effect of Yoga Therapy on Cardiac Function and N Terminal Pro BNP in Heart Failure." in: Integrative medicine insights, Vol. 9, pp. 1-6, (2014) (PubMed).

    Fuernau, Poenisch, Eitel, de Waha, Desch, Schuler, Adams, Werdan, Zeymer, Thiele: "Growth-differentiation factor 15 and osteoprotegerin in acute myocardial infarction complicated by cardiogenic shock: a biomarker substudy of the IABP-SHOCK II-trial." in: European journal of heart failure, Vol. 16, Issue 8, pp. 880-7, (2014) (PubMed).

    Kara, Tonbul, Karabel, Akca, Uras, Tatli: "The role of serum N-terminal pro-brain natriuretic peptide in transient tachypnea of the newborn." in: European review for medical and pharmacological sciences, Vol. 17, Issue 13, pp. 1824-9, (2013) (PubMed).

  • Target Alle NT-ProBNP ELISA Kits anzeigen
    NT-ProBNP (Pro-Brain Natriuretic Peptide (NT-ProBNP) (NT-ProBNP))
    Andere Bezeichnung
    Pro-Brain Natriuretic Peptide (NT-ProBNP) (NT-ProBNP Produkte)
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